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Originally published as Genetics Published Articles Ahead of Print on August 24, 2008.
Genetics, Vol. 180, 27-39, September 2008, Copyright © 2008
doi:10.1534/genetics.108.089243
Rtf1-Mediated Eukaryotic Site-Specific Replication Termination
T. Eydmann*,
E. Sommariva*,
T. Inagawa*,
S. Mian
,
A. J. S. Klar
and
J. Z. Dalgaard*,1
* Marie Curie Research Institute, The Chart, Oxted RH8 0TL, United Kingdom, Lawrence Berkeley National Laboratory, Life Sciences Division, Berkeley, California 94720-8265 and National Cancer Institute, Gene Regulation and Chromosome Biology Laboratory, Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201
1 Corresponding author: Marie Curie Research Institute, The Chart, Oxted RH8 OTL, Surrey, UK.
E-mail: j.dalgaard{at}mcri.ac.uk
The molecular mechanisms mediating eukaryotic replication termination and pausing remain largely unknown. Here we present the molecular characterization of Rtf1 that mediates site-specific replication termination at the polar Schizosaccharomyces pombe barrier RTS1. We show that Rtf1 possesses two chimeric myb/SANT domains: one is able to interact with the repeated motifs encoded by the RTS1 element as well as the elements enhancer region, while the other shows only a weak DNA binding activity. In addition we show that the C-terminal tail of Rtf1 mediates self-interaction, and deletion of this tail has a dominant phenotype. Finally, we identify a point mutation in Rtf1 domain I that converts the RTS1 element into a replication barrier of the opposite polarity. Together our data establish that multiple protein DNA and protein–protein interactions between Rtf1 molecules and both the repeated motifs and the enhancer region of RTS1 are required for site-specific termination at the RTS1 element.