A Bacterial Artificial Chromosome-Based Genetic Linkage Map of the Nematode Pristionchus pacificus

Corresponding author: Ralf J. Sommer, Max-Planck Institute for Developmental Biology, Spemannstrasse 37, D-72076 Tübingen, Germany., ralf.sommer{at}tuebingen.mpg.de (E-mail)

Communicating editor: D. KINGSLEY

	ABSTRACT

TOP ABSTRACT MATERIALS AND METHODS RESULTS AND DISCUSSION LITERATURE CITED

To understand the evolution of developmental processes, nonmodel organisms in the nematodes, insects, and vertebrates are compared with established model systems. Often, these comparisons suffer from the inability to apply sophisticated technologies to these nonmodel species. In the nematode Pristionchus pacificus, cellular and genetic analyses are used to compare vulva development to that of Caenorhabditis elegans. However, substantial changes in gene function between P. pacificus and C. elegans limit the use of candidate gene approaches in studying P. pacificus mutations. To facilitate map-based cloning of mutations in P. pacificus, we constructed a BAC-based genetic linkage map. A BAC library of 13,440 clones was generated and completely end sequenced. By comparing BAC end and EST sequences between the "wild-type" strain P. pacificus var. California and the polymorphic strain P. pacificus var. Washington, 133 single-stranded conformational polymorphisms were identified. These markers were tested on a meiotic mapping panel of 46 randomly picked F₂ animals after a cross of the two strains, providing the first genetic linkage map of P. pacificus. A mapping strategy using two selected markers per chromosome was devised and the efficiency of this approach was illustrated by the mapping of the Ppa-unc-1/Twitchin gene.

RECENT advances in mapping and sequencing genomes are revolutionizing our knowledge of several model organisms including humans. These new technologies not only speed our understanding of model systems, but also bring together disparate branches and problems of biology. In evolutionary biology, for instance, a long-standing question is how changes in developmental processes translate into morphological diversity (RAFF 1996 ; GERHART and KIRSCHNER 1997 ). However, a detailed genetic and molecular understanding of developmental processes is currently restricted to a small number of model organisms such as Caenorhabditis elegans, Drosophila, or mouse. The availability of new DNA technologies will help extend our knowledge of nonmodel organisms and will facilitate the comparison of their development with that of phylogenetically related model systems.

Pristionchus pacificus is a free-living nematode of the Diplogastridae family and has recently been developed as a satellite organism for functional comparative studies in developmental biology (EIZINGER et al. 1999 ; SOMMER 2000 ). Like the model organism C. elegans, P. pacificus can be cultured easily in the laboratory. It has a 4-day life cycle and propagates as self-fertilizing hermaphrodites or by outcrossing after the spontaneous generation of males (SOMMER et al. 1996 ). Many cellular, genetic, and molecular techniques successfully used in C. elegans are also applicable to P. pacificus, generating the basis for studying evolutionary developmental biology.

A developmental process studied in great detail is the development of the vulva, the egg-laying structure of nematodes. Comparative studies of vulva development between C. elegans and P. pacificus indicated that although homologous precursor cells are involved in vulva formation, the cell-cell interactions required to form a proper vulva vary greatly between both species (SOMMER 2001 ). Furthermore, the comparison of mutations in the homeotic genes lin-39, mab-5, and vab-7 in both species revealed substantial changes in the function of these genes during vulva formation (EIZINGER and SOMMER 1997 ; JUNGBLUT and SOMMER 1998 , JUNGBLUT and SOMMER 2001 ; SOMMER et al. 1998 ). Thus, a candidate gene approach or other techniques relying on C. elegans knowledge might be uninformative or even misleading in studying P. pacificus mutations. To overcome such limitations, map-based cloning offers an ideal alternative. Here, we describe a bacterial artificial chromosome (BAC)-based genetic linkage map of P. pacificus that allows map-based cloning of genes in this organism. A mapping strategy using two selected single-strand conformation polymorphism (SSCP) markers per chromosome has been devised and the efficiency of this approach is illustrated by the mapping of the Ppa-unc-1/Twitchin gene.

	MATERIALS AND METHODS

TOP ABSTRACT MATERIALS AND METHODS RESULTS AND DISCUSSION LITERATURE CITED

BAC library construction:
Agarose plugs containing high-molecular-weight DNA were prepared from first larval stage worms from synchronized cultures as described previously (OSOEGAWA et al. 1998 , OSOEGAWA et al. 2000 ). Plugs were partially digested with HindIII and size fractioned in two steps by pulsed-field gel electrophoresis. Several fractions of size-selected DNA fragments were electroeluted and cloned into the vector pIndigoBAC-536 (SHIZUYA et al. 1992 ). The ligation product was electroporated into Escherichia coli DH10B cells. After NotI sizing 96 white clones of each fraction, the four best fractions were picked by robot.

BAC end sequencing:
To prepare BAC DNA for end sequencing, 4 ml of 2x YT medium-chloramphenicol (12.5 µg/µl) was inoculated with 4 µl of BAC freezer stocks, and cultures were grown for 20 hr at 37°. BAC-DNA was then prepared using the R.E.A.L. kit (QIAGEN, Valencia, CA) on a robotic platform (BioRobot 8000). Sequencing reactions were set up according to manufacturer's instructions for the Big Dye Terminator chemistry (Applied Biosystems, Foster City, CA). Samples were analyzed using capillary electrophoresis (Applied Biosystems, ABI Prism 3700). Base calling was performed by the software PHRED and vector sequences were masked with CrossMatch. Sequences containing at least 100 nonvector bases with Phred values >20 were used for further analysis. All programs are running under Solaris 5.7 on a Sun Sparc Ultra 10 workstation. The sequences are housed in a MySQL (v.3.23.33) database and are accessible online. The GenBank accession numbers of the sequences are 3576383–3595739. The program PrimeArray (RADDATZ et al. 2001 ) was used to design primers for testing conformational polymorphisms.

Preparation of meiotic mapping panel and PCR amplification:
The meiotic mapping panel was prepared from 46 randomly picked F₂ animals from a cross between phenotypically marked hermaphrodites of the California strain and Washington strain males. F₂ animals were cloned and genomic DNA was extracted from them using the Bio-Rad (Richmond, CA) genomic DNA extraction kit. For a typical PCR assay, 1/1000 of this DNA preparation was used as a template. PCR amplification was pipetted in 96-well plates on a Beckman (Fullerton, CA) Biomek robot in 20-µl reaction volumes. A typical PCR reaction mixture consisted of 10 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl₂, 200 µM dNTP, 1 unit Taq polymerase, and 1 µM of each primer. Thermocycling was done in a Perkin-Elmer (Norwalk, CT) Gene Amp 9700 PCR machine under standard conditions consisting of an initial denaturation at 94° for 3 min; followed by 30 cycles of 94° for 1 min, 50° for 1 min, and 72° for 1 min; and a final incubation at 72° for 7 min. The PCR products were first confirmed on a 1% agarose gel before they were loaded onto SSCP gels.

Gel electrophoresis and SSCP detection:
For SSCP detection, the samples were diluted 1:1 in denaturing solution (95% formamide, 0.1% xylene cyanol, 0.1% bromophenol blue, and 10 mM NaOH), denatured at 95° for 5 min, and loaded onto a GeneGel Excel prepoured 6% acrylamide gel (Pharmacia Biotech, Piscataway, NJ). Gels were fixed and silver stained to detect the DNA. To count for polymorphisms, mobility differences between the California and Washington PCR products were checked at both the single- and the double-stranded DNA levels. Wherever polymorphisms were detected between the two strains, the Washington bands were sequenced. Both the sequences were assembled using the program Sequencher and the differences were tabulated. Ninety-three of the polymorphisms were characterized at the sequence level.

Data collection and linkage analysis:
Data were collected using Map Manager software (http://mcbio.med.buffalo.edu/mapmgr.html) and analysis was conducted with Map Manager and MAPMAKER software (LANDER et al. 1987 ). Each locus was placed initially at the position that maximized its LOD score as indicated by the "Make Linkage Groups" command of Map Manager. Local order was then determined by manually placing the marker at the location that minimized the number of double recombinants. Data analysis ordered 122 loci into six linkage groups that were supported by LOD scores 5. Map graphics were created with MAPMAKER using the Kosambi mapping function and later on modified for clarity with FreeHand 9.0.

	RESULTS AND DISCUSSION

TOP ABSTRACT MATERIALS AND METHODS RESULTS AND DISCUSSION LITERATURE CITED

BAC library formation and BAC end sequencing:
We constructed a BAC library of P. pacificus var. California in the pIndigoBAC-536 vector using HindIII partial digests (SHIZUYA et al. 1992 ). In total, 13,440 BAC clones were generated with insert sizes ranging from 100 to 180 kb and an average insert size of 128 kb (Fig 1A). All BAC clones were sequenced from both ends, yielding 18,133 good-quality sequences, 1400 of which have similarities to known C. elegans sequences. All sequences are accessible via GenBank (see MATERIALS AND METHODS).

View larger version (61K): In this window In a new window Download PPT slide   Figure 1. (A) Insert size distribution of 192 randomly picked BAC clones. (B) Strategy for SSCP marker detection. PCR primers were designed to amplify a 180- to 200-bp fragment in BAC end sequences. California and Washington amplicons were tested for polymorphisms using the SSCP technique.

Polymorphisms generated by SSCP analysis:
Previous studies revealed substantial polymorphisms between various strains of P. pacificus (SCHLAK et al. 1997 ; SRINIVASAN et al. 2001 ). The highest degree of polymorphisms was observed between the wild-type strain from Pasadena, California and the strain from Port Angeles, Washington (SRINIVASAN et al. 2001 ). Our strategy involved designing PCR primers to randomly amplify a 180- to 200-bp amplicon (Fig 1B) in a subset of the sequenced BAC ends using PrimeArray (RADDATZ et al. 2001 ). The resulting PCR fragments were analyzed for the presence of polymorphisms using the SSCP technique (ORITA et al. 1989 ). From the 480 PCR reactions performed, 93 resulted in SSCP markers. These were confirmed by comparing the sequences of the PCR products of both strains. We observed 260 substitutions (73.7%) and 93 insertion/deletions (indels; 26.3%). C. elegans has slightly higher substitution rates (75.1%) and correspondingly lower indels (24.9%) between the most variable strains (KOCH et al. 2000 ; WICKS et al. 2001 ). Of the observed substitutions in P. pacificus, 47.3% were transitions and 52.7% were transversions, which is exactly the converse of that in C. elegans (KOCH et al. 2000 ; WICKS et al. 2001 ).

The BAC-end-based SSCP analysis was complemented by a search for SSCP markers in a subset of the existing P. pacificus expressed sequence tag (EST) clones and previously cloned vulval patterning genes. In a total of 119 ESTs and genes tested by SSCP analysis, 40 (33%) were polymorphic. Together, 133 SSCP markers were generated from both BAC end and EST sequences.

A genetic linkage map of P. pacificus:
To construct a genetic linkage map, we scored the 133 SSCP markers on a meiotic mapping panel. After crossing a genetically marked California hermaphrodite with Washington males, 46 random F₂ individuals were cloned, constituting the meiotic mapping panel. Linkage analyses assembled 122 of the 133 SSCP markers on a map with six linkage groups, which corresponds to the observation of six chromosomes after 4',6-diamidino-2-phenylindole (DAPI) staining of P. pacificus oocytes (Fig 2; SOMMER et al. 1996 ). All map positions were supported by LOD scores 5 and there were no gaps 20 cM (Fig 2). The remaining unassigned SSCPs are mostly unlinked to one another. The 122 assigned SSCP markers occupied 88 unique map positions. In total, the linkage groups spanned 338.6 cM.

View larger version (66K): In this window In a new window Download PPT slide   Figure 2. A genetic linkage map of P. pacificus. DNA samples were prepared from 46 randomly picked F2 progeny of an original cross of a hermaphrodite of the California strain and a male from the Washington strain. Amplified fragments were separated on SSCP gels. Linkage groups were identified with the program Map Manager (Stanford University). All map positions are supported by LOD scores 5, using the Kosambi mapping function to estimate the occurrence of double crossovers. Asterisk indicates representative SSCP markers for mapping mutants to particular chromosomes.

Mapping of Ppa-unc-1:
Our SSCP-based genetic linkage map provides a unique source of information to facilitate mapping of experimentally derived mutations in P. pacificus. To demonstrate the efficiency of map-based cloning, we mapped Ppa-unc-1, which has previously been suggested to represent the homolog of Cel-unc-22/Twitchin based on the unique phenotype of mutant animals (Fig 3, a and b; SOMMER et al. 1996 ). California-derived mutant hermaphrodites were crossed with Washington males and 21 clones of mutant F₂ animals were established (Fig 3C). Two representative SSCP markers were selected per chromosome (Fig 2). The selected markers were tested on these 21 clones for segregation of the paternal Washington (W) vs. maternal California (C) pattern. The segregation was measured as a unitless map ratio, W/C. Unlinked markers are expected to be represented equally and should have a ratio of 1. In contrast, linked markers have a predominant maternal segregation pattern and therefore yield a value close to 0. Our analysis of Washington-backcrossed Ppa-unc-1 animals showed clear linkage to chromosome IV (Fig 3D). Specifically, all 21 clones segregated the maternal pattern of the marker S148 (Fig 3D).

View larger version (61K): In this window In a new window Download PPT slide   Figure 3. (A and B) Photographs of P. pacificus wild-type (A) and Ppa-unc-1 mutant animals (B). (C) Crossing scheme for obtaining Washington-backcrossed mutant animals for mapping. (D) Chromosome localization for Ppa-unc-1. One of the two markers on chromosome IV gives a 0 W/C ratio (*).

To verify this result, we cloned a genomic fragment of the Cel-unc-22/Twitchin ortholog from P. pacificus and generated an SSCP marker in this gene fragment. We mapped the Ppa-unc-1-obtained SSCP marker on the genetic linkage map and found that it had a position identical to S148 (Fig 2). This result confirms that Ppa-unc-1 represents the homolog of Cel-unc-22 and also indicates that P. pacificus mutations can be successfully mapped using the existing genetic linkage map.

Mapping of the P. pacificus X chromosome:
Previous studies revealed that P. pacificus males are induced by meiotic nondisjunction of the X chromosome (SOMMER et al. 1996 ). To identify which of the linkage groups corresponds to the X chromosome, we looked for linkage of the representative SSCP markers to the X-linked genetic marker Ppa-unc-14 (SOMMER et al. 1996 ). California-derived mutant hermaphrodites were crossed with Washington males and F₁ males were pooled for further analysis (Fig 4A). These males are heterozygous for all autosomes but hemizygous for the California-derived X chromosome. S105 and other SSCP markers on the same chromosome segregated only the maternal pattern, thereby specifying this chromosome as the X chromosome (Fig 4B).

View larger version (26K): In this window In a new window Download PPT slide   Figure 4. (A) Crossing scheme for obtaining hemizygous F1 males. (B) SSCP analysis of hemizygous F1 males with the marker S105. Only the California variant was amplified, indicating that S105 represents an X-chromosomal marker. Other SSCP markers tested were S140 and S155. M, pooled F1 males; C, W, and H, California, Washington, and heterozygous control DNAs, respectively.

Comparison to C. elegans:
This study provides a genetic linkage map of P. pacificus and lays the foundation for positional cloning. We mapped 133 SSCP markers on a meiotic mapping panel composed of 46 randomly picked F₂ P. pacificus animals. The average interval between markers is between 2 and 3 cM/SSCP and is similar on all linkage groups. However, it remains unknown if these SSCP markers provide an equal representation of the genome. As most SSCP markers are BAC derived, a potential underrepresentation of certain chromosomal regions in the BAC library might cause these regions to be excluded from the map.

A comparison of the genetic linkage map of P. pacificus with that of C. elegans indicates no overall synteny between the P. pacificus and the C. elegans genomes. For example, the cell death regulator ced-3 is located on chromosome IV in C. elegans together with unc-22 and let-60. In contrast, Ppa-ced-3 (S173) is located on chromosome II, whereas Ppa-unc-1 and Ppa-let-60 (S2) are located on chromosome IV (Fig 2). Similar results have been obtained during mapping and cloning of vulval patterning genes (J. SRINIVASAN and R. J. SOMMER, unpublished observation). However, our analysis does not rule out the existence of small-scale synteny, which will be revealed by future sequencing efforts. These findings are in agreement with studies on C. elegans and C. briggsae, also indicating a violation of synteny (KENT and ZAHLER 2000 ).

Another striking feature of the C. elegans genome is the central clustering of genes (C. ELEGANS SEQUENCING CONSORTIUM 1998). However, as most SSCP markers of P. pacificus were generated from BAC ends, no exact information is available on the distribution of genes along the chromosomes. Similarly, the relationship between the recombination rates and gene density and the gene number per chromosome awaits further analysis.

Toward an integrated genome map of P. pacificus:
Our genetic linkage map is built mostly on BAC-end-derived SSCP markers. Currently, this study is complemented by a physical mapping project of the complete BAC library using amplified fragment length polymorphism fingerprinting (T. JESSE, M. DE BOTH and R. J. SOMMER, unpublished data). Contigs generated in the physical map project can be anchored on the genetic linkage map by generating one SSCP marker per contig and testing it on the mapping panel. Together, the genetic and physical maps will provide an integrated genome map of P. pacificus because both are BAC clone based, a feature that allows the compatibility of BAC markers and clones used in both projects.

Furthermore, this work establishes P. pacificus as the primary candidate for comparative nematode genomics, with the long-term goal of sequencing its genome and comparing it to C. elegans. The comparison of the genome sequence of two distantly related free-living nematodes is also a prerequisite for studying and understanding the genome sequence of parasitic nematodes, many of which cause major diseases in humans and animals.

Manuscript received March 12, 2002; Accepted for publication May 24, 2002.

	LITERATURE CITED

TOP ABSTRACT MATERIALS AND METHODS RESULTS AND DISCUSSION LITERATURE CITED

Genome sequence of the nematode C. elegans: a platform for investigating biology. (1998) Science 282:2012-2018.[Abstract/Free Full Text]

EIZINGER, A. and R. J. SOMMER, 1997  The homeotic gene lin-39 and the evolution of nematode epidermal cell fates. Science 278:452-455.[Abstract/Free Full Text]

EIZINGER, A., B. JUNGBLUT, and R. J. SOMMER, 1999  Evolutionary change in the functional specificity of genes. Trends Genet. 15:197-202.[Medline]

GERHART, J., and M. KIRSCHNER, 1997 Cells, Embryos and Evolution. Blackwell Scientific, Oxford.

JUNGBLUT, B. and R. J. SOMMER, 1998  The Pristionchus pacificus mab-5 gene is involved in the regulation of ventral epidermal cell fates. Curr. Biol. 8:775-778.[Medline]

JUNGBLUT, B. and R. J. SOMMER, 2001  The nematode even-skipped homolog vab-7 regulates gonad and vulva position in Pristionchus pacificus.. Development 128:253-261.[Abstract]

KENT, W. J. and A. M. ZAHLER, 2000  Conservation, regulation, synteny, and introns in a large-scale C. briggsae-C. elegans genomic alignment. Genome Res. 10:1115-1125.[Abstract/Free Full Text]

KOCH, R., H. G. VAN LUENEN, M. VAN DER HORST, K. L. THIJSSEN, and R. H. PLASTERK, 2000  Single nucleotide polymorphisms in wild isolates of Caenorhabditis elegans.. Genome Res. 10:1690-1696.[Abstract/Free Full Text]

LANDER, E. S., P. GREEN, J. ABRAHAMSON, A. BARLOW, and M. J. DALY et al., 1987  MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174-181.[Medline]

ORITA, M., Y. SUZUKI, T. SEKIYA, and K. HAYASHI, 1989  Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5:874-879.[Medline]

OSOEGAWA, K., P. Y. WOON, B. ZHAO, E. FRENGEN, and M. TATENO et al., 1998  An improved approach for construction of bacterial artificial chromosome libraries. Genomics 52:1-8.[Medline]

OSOEGAWA, K., M. TATENO, P. Y. WOON, E. FRENGEN, and A. G. MAMMOSER et al., 2000  Bacterial artificial chromosome libraries for mouse sequencing and functional analysis. Genome Res. 10:116-128.[Abstract/Free Full Text]

RADDATZ, G., M. DEHIO, T. F. MEYER, and C. DEHIO, 2001  PrimeArray: genome-scale primer design for DNA-microarray construction. Bioinformatics 17:98-99.[Abstract/Free Full Text]

RAFF, R., 1996 The Shape of Life. The University of Chicago Press, Chicago.

SCHLAK, I., A. EIZINGER, and R. J. SOMMER, 1997  High rate of restriction fragment length polymorphisms between two populations of the nematode Pristionchus pacificus (Diplogastridae). J. Zool. Syst. Evol. 35:137-142.

SHIZUYA, H., B. BIRREN, U. J. KIM, V. MANCINO, and T. SLEPAK et al., 1992  Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89:8794-8797.[Abstract/Free Full Text]

SOMMER, R. J., 2000  Evolution of nematode development. Curr. Opin. Genet. Dev. 10:443-448.[Medline]

SOMMER, R. J., 2001  As good as they get: cells in nematode vulva development and evolution. Curr. Opin. Cell Biol. 13:715-720.[Medline]

SOMMER, R. J., L. K. CARTA, A. S.-Y. KIM, and P. W. STERNBERG, 1996  Morphological, genetic and molecular description of Pristionchus pacificus sp. n. (Nematoda: Neodiplogastridae). Fundam. Appl. Nematol. 19:511-521.

SOMMER, R. J., A. EIZINGER, K. Z. LEE, B. JUNGBLUT, and A. BUBECK et al., 1998  The Pristionchus HOX gene Ppa-lin-39 inhibits programmed cell death to specify the vulva equivalence group and is not required during vulval induction. Development 125:3865-3873.[Abstract]

SRINIVASAN, J., A. PIRES-DASILVA, A. GUTIERREZ, M. ZHENG, and B. JUNGBLUT et al., 2001  Microevolutionary analysis of the nematode genus Pristionchus suggests a recent evolution of redundant developmental mechanisms during vulva formation. Evol. Dev. 3:229-240.[Medline]

WICKS, S. R., R. T. YEH, W. R. GISH, R. H. WATERSTON, and R. H. PLASTERK, 2001  Rapid gene mapping in Caenorhabditis elegans using a high density polymorphism map. Nat. Genet. 28:160-164.[Medline]

This article has been cited by other articles:

				R. Rae, B. Schlager, and R. J. Sommer Pristionchus pacificus: A Genetic Model System for the Study of Evolutionary Developmental Biology and the Evolution of Complex Life-History Traits CSH Protocols, October 1, 2008; 2008(11): pdb.emo102 - pdb.emo102. [Abstract] [Full Text]

				R. L. Hong, H. Witte, and R. J. Sommer Natural variation in Pristionchus pacificus insect pheromone attraction involves the protein kinase EGL-4 PNAS, June 3, 2008; 105(22): 7779 - 7784. [Abstract] [Full Text] [PDF]

				B. Yi and R. J. Sommer The pax-3 gene is involved in vulva formation in Pristionchus pacificus and is a target of the Hox gene lin-39 Development, September 1, 2007; 134(17): 3111 - 3119. [Abstract] [Full Text] [PDF]

				H. Zauner and R. J. Sommer Evolution of robustness in the signaling network of Pristionchus vulva development PNAS, June 12, 2007; 104(24): 10086 - 10091. [Abstract] [Full Text] [PDF]

				C. Dieterich, W. Roeseler, P. Sobetzko, and R. J. Sommer Pristionchus.org: a genome-centric database of the nematode satellite species Pristionchus pacificus Nucleic Acids Res., January 12, 2007; 35(suppl_1): D498 - D502. [Abstract] [Full Text] [PDF]

				J.-T. A. Chiang, M. Steciuk, B. Shtonda, and L. Avery Evolution of pharyngeal behaviors and neuronal functions in free-living soil nematodes J. Exp. Biol., May 15, 2006; 209(10): 1859 - 1873. [Abstract] [Full Text] [PDF]

				A. Gutierrez and R. J. Sommer Evolution of dnmt-2 and mbd-2-like genes in the free-living nematodes Pristionchus pacificus, Caenorhabditis elegans and Caenorhabditis briggsae Nucleic Acids Res., December 2, 2004; 32(21): 6388 - 6396. [Abstract] [Full Text] [PDF]

				A. Pires-daSilva and R. J. Sommer Conservation of the global sex determination gene tra-1 in distantly related nematodes Genes & Dev., May 15, 2004; 18(10): 1198 - 1208. [Abstract] [Full Text] [PDF]

				J. Srinivasan, G. W. Otto, U. Kahlow, R. Geisler, and R. J. Sommer AppaDB: an AcedB database for the nematode satellite organism Pristionchus pacificus Nucleic Acids Res., January 1, 2004; 32(90001): D421 - 422. [Abstract] [Full Text] [PDF]

				K.-Z. Lee and R. J. Sommer Operon Structure and Trans-Splicing in the Nematode Pristionchus pacificus Mol. Biol. Evol., December 1, 2003; 20(12): 2097 - 2103. [Abstract] [Full Text] [PDF]

				K.-Z. Lee, A. Eizinger, R. Nandakumar, S. C. Schuster, and R. J. Sommer Limited microsynteny between the genomes of Pristionchus pacificus and Caenorhabditis elegans Nucleic Acids Res., May 15, 2003; 31(10): 2553 - 2560. [Abstract] [Full Text] [PDF]