Suppressors of mdm20 in Yeast Identify New Alleles of ACT1 and TPM1 Predicted to Enhance Actin-Tropomyosin Interactions

Corresponding author: Janet M. Shaw, Department of Biology, University of Utah, 257 S. 1400 E., Salt Lake City, UT 84112-0840., shaw{at}bioscience.utah.edu (E-mail)

Communicating editor: M. D. ROSE

	ABSTRACT

TOP ABSTRACT MATERIALS AND METHODS RESULTS DISCUSSION LITERATURE CITED

The actin cytoskeleton is required for many aspects of cell division in yeast, including mitochondrial partitioning into growing buds (mitochondrial inheritance). Yeast cells lacking MDM20 function display defects in both mitochondrial inheritance and actin organization, specifically, a lack of visible actin cables and enhanced sensitivity to Latrunculin A. mdm20 mutants also exhibit a temperature-sensitive growth phenotype, which we exploited to isolate second-site suppressor mutations. Nine dominant suppressors selected in an mdm20/mdm20 background rescue temperature-sensitive growth defects and mitochondrial inheritance defects and partially restore actin cables in haploid and diploid mdm20 strains. The suppressor mutations define new alleles of ACT1 and TPM1, which encode actin and the major form of tropomyosin in yeast, respectively. The ACT1 mutations cluster in a region of the actin protein predicted to contact tropomyosin, suggesting that they stabilize actin cables by enhancing actin-tropomyosin interactions. The characteristics of the mutant ACT1 and TPM1 alleles and their potential effects on protein structure and binding are discussed.

IN mitotically dividing yeast cells, the actin cytoskeleton is organized into two different types of filament-based structures, cortical actin patches that cluster in the growing daughter cell (bud) and polarized actin cables that align along the mother-bud axis (BOTSTEIN et al. 1997 ). Actin patches and cables perform numerous functions required for the generation of new buds and the subsequent events of cell division (BOTSTEIN et al. 1997 ). At the beginning of the cell cycle, actin is required for both bud site selection and bud emergence. The actin cytoskeleton also plays a central role in the establishment of cell polarity, which in turn sets up directed secretion and growth of the bud. Proper mitotic spindle orientation requires actin function. Finally, actin is a component of the actomyosin ring, which plays a critical role in cytokinesis.

Recent studies indicate that actin is also involved in the inheritance of cellular organelles such as vacuoles (HILL et al. 1996 ; CATLETT and WEISMAN 1998 ; WANG et al. 1998 ) and mitochondria (DRUBIN et al. 1993 ; LAZZARINO et al. 1994 ; SIMON et al. 1995 , SIMON et al. 1997 ; HERMANN et al. 1997 ; BOLDOGH et al. 1998 ; HERMANN and SHAW 1998 ) during yeast budding. Treatment with the actin-disrupting drug, Latrunculin A (Lat-A), and mutations in the ACT1 gene have been shown to block the inheritance of mitochondria (SIMON et al. 1995 ; BOLDOGH et al. 1998 ) and vacuoles (HILL et al. 1996 ) during division. In addition, a type V myosin, Myo2p, is proposed to be a component of the vacuole inheritance machinery (HILL et al. 1996 ; CATLETT and WEISMAN 1998 ). Although none of the known yeast myosins appear to be essential for mitochondrial inheritance (SIMON et al. 1995 ), mitochondrial membranes are found closely aligned with actin cables in vivo (DRUBIN et al. 1993 ) and purified mitochondria exhibit ATP-dependent binding and transport along stabilized actin filaments in vitro (LAZZARINO et al. 1994 ; SIMON et al. 1995 ).

Studies of the yeast MDM20 gene provide additional support for the role of actin cables in mitochondrial transport. MDM20 encodes a low abundance 93-kD cytoplasmic protein required for efficient mitochondrial inheritance at all temperatures (HERMANN et al. 1997 ; HERMANN 1998 ). At elevated temperatures (>34°), MDM20 becomes essential for growth (HERMANN 1998 ). The mitochondrial inheritance defect in mdm20 mutants appears to result from a primary defect in actin organization since these cells also lack visible actin cables (HERMANN et al. 1997 ). The actin cable defect in mdm20 cells is similar to that observed in tpm1 mutants lacking the actin filament-binding protein, tropomyosin 1 (LIU and BRETSCHER 1989 ). In muscle cells, tropomyosin regulates myosin-actin interactions during muscle contraction. In nonmuscle cells like yeast, tropomyosin is thought to function in the assembly and/or stabilization of actin cables. Although TPM1 and MDM20 encode proteins with unrelated structures, extra copies of TPM1 suppress actin cable and mitochondrial inheritance defects caused by mdm20, and tpm1 and mdm20 mutations are synthetically lethal (HERMANN et al. 1997 ). In addition, MDM20 and TPM1 display a similar pattern of genetic interactions; mutations in both genes result in synthetic lethal phenotypes when combined with mutations in BEM2 (encodes a GTPase activating protein required for bud emergence; BENDER and PRINGLE 1991 ; PETERSON et al. 1994 ) and MYO2 (encodes a type V myosin; JOHNSTON et al. 1991 ; CHENEY et al. 1993 ). These data suggest that Mdm20p and Tpm1p act in the same or parallel pathways to control the assembly or stabilization of actin cables.

Interactions between a large number of different genes (including TPM1, MYO2, and BEM2) have been shown to play a role in yeast actin assembly and function (LIU and BRETSCHER 1992 ; WANG and BRETSCHER 1995 ; BOTSTEIN et al. 1997 ). In an effort to determine how MDM20 fits into this network of genes, we selected dominant suppressors of the mdm20 temperature-sensitive growth defect. The suppressor mutations we recovered all fall within the ACT1 and TPM1 genes. Our findings raise the possibility that Mdm20p cooperates with the most central elements of actin cables, perhaps modulating the interaction between actin and tropomyosin.

	MATERIALS AND METHODS

TOP ABSTRACT MATERIALS AND METHODS RESULTS DISCUSSION LITERATURE CITED

Yeast strains, media, and genetic techniques:
Saccharomyces cerevisiae strains used in this study are derived from the FY strain background (WINSTON et al. 1995 ) and are listed in Table 1. Standard yeast genetic techniques were used (SHERMAN et al. 1986 ). Rich medium (YPDextrose) and synthetic medium (SDextrose) were prepared as described (SHERMAN et al. 1986 ). High salt medium used to detect osmotic sensitivity was made by supplementing synthetic medium with 0.9 M NaCl (CHOWDHURY et al. 1992 ).

Isolation of suppressor mutations:
The diploid strains JSY1371 and JSY1372 (Table 1) were used to obtain pseudorevertants and are homozygous for mdm20 and mdm20-1, respectively. The mdm20 disruption eliminates 740 of 796 codons of the MDM20 open reading frame (ORF; HERMANN et al. 1997 ). mdm20-1 is also a severe loss-of-function allele but, unlike mdm20, permits the expression of a largely intact protein. It contains two single base pair deletions in codons 749 and 750 of the predicted coding region, resulting in a frameshift that introduces a premature stop at codon 750 (HERMANN 1998 ). Both mdm20 and mdm20-1 cause temperature-sensitive lethality at 37°.

To obtain dominant pseudorevertants, we selected spontaneous suppressors arising in the two mdm20/mdm20 strains described above. Following the method of ADAMS and BOTSTEIN 1989 , multiple, independently inoculated 2-ml cultures of JSY1371 or JSY1372 were grown overnight at the permissive temperature (25°). Each individual culture was used to seed a single YPD plate with approximately 3 x 10⁶ cells. After 4 days at the nonpermissive temperature (37°), plates were examined for spontaneous pseudorevertants. Temperature-resistant colonies (1 from each plate) were isolated and retested for suppression of the 37° growth defect, while at the cellular level, the strains were examined for changes in their actin organization and mitochondrial inheritance phenotypes (see below). The pseudorevertants were also checked for new phenotypes including cold sensitivity, Lat-A sensitivity, and salt/osmotic sensitivity. Suppressor mutations isolated in the homozygous mdm20-1/mdm20-1 strain were tested for their ability to suppress the mdm20 disruption allele (mdm20).

Genetic and molecular characterization of suppressor mutations:
Recombination frequencies were used to evaluate the potential linkage of mdm20 suppressor mutations to the ACT1 and TPM1 loci. To generate a marked strain for the ACT1 linkage analysis, the HIS3 selectable marker was integrated between the ACT1 and YPT1 loci in JSY1340 (genotypically identical to JSY3094, Table 1), creating JSY3079 (Table 1). Correct integration into the 5'-flanking DNA of the ACT1 locus was verified by PCR, using a primer pair that hybridized to the HIS3 and ACT1 coding sequences. The TPM1 linkage analysis was performed with strain JSY1081, containing a HIS3 disruption of the TPM1 coding region (HERMANN et al. 1997 ; the original disruption strain is JSY707, isogenic to JSY1081, Table 1).

The existence of mutations at the TPM1 or ACT1 loci in mdm20 suppressor strains was verified by DNA sequencing. Genomic DNA was isolated from each mdm20 suppressor mutant, and the ORF plus 475 bp of flanking DNA were PCR amplified from each gene. Purified amplification products were sequenced by the University of Utah DNA sequencing facility. Sequence analysis was performed using DNA* software (DNAStar, Madison, WI).

To verify the DNA sequence prediction that the DMT2-3/TPM1-5 mutation causes seven amino acids to be added the Tpm1p N terminus, mutant Tpm1 protein was purified from strain JSY3315 as previously described (DREES et al. 1995 ) and partially sequenced. Fourteen cycles of Edman degradation were performed by the University of Utah DNA and peptide facility.

Phenotypic characterization of suppressor mutations:
Growth phenotypes of mutant and wild-type strains were compared by a serial dilution assay. Overnight cultures grown in YPD were diluted in fresh medium and grown to midlog phase. The cells were washed once and diluted in series to concentrations ranging from 1.3 x 10⁷ cells/ml to 165 cells/ml. After spotting 5 µl of each dilution onto YPD medium, identical plates were incubated for 3 days at 25° and 37°.

The actin cytoskeleton in mdm20 suppressor strains was visualized with 0.5 µM Alexa568 phalloidin (Molecular Probes, Eugene, OR) following the method of ADAMS and PRINGLE 1991 with the exception that fixed cells were stained on ice for 1 hr as suggested by KARPOVA et al. 1998 . Stained and washed cells were maintained on ice until mounted for microscopy. Actin cables are most easily observed in cells with small to medium buds. Therefore, only cells in this stage were scored for the presence or absence of cable(-like) structures.

Mitochondrial inheritance phenotypes were quantified as described previously (HERMANN et al. 1997 ; ROEDER et al. 1998 ) in mdm20 suppressor strains transformed with the pDO12 plasmid (OTSUGA et al. 1998 ), which expresses a mitochondrial matrix-targeted Cox4-green fluorescent protein (GFP) fusion protein. Overnight cultures grown at 25° in synthetic medium were diluted to an OD₆₀₀ of 1.0 in fresh medium and grown an additional 2–3 hr at 25° or 37° before scoring. Digital microscopic images of cells were acquired as described previously (OTSUGA et al. 1998 ).

The Lat-A halo assay was performed essentially as described by AYSCOUGH et al. 1997 except that plates were seeded with a fixed number of cells (2.25 x 10⁶) instead of a fixed volume of cell culture. Digital images of each plate were acquired and halo dimensions were determined using the application NIH Image V1.61 (http://rsb.info.nih.gov/nih-image). Two wild-type strains, JSY840 and JSY999 (Table 1), were used separately or together in independent repetitions of the experiment as standards for Lat-A sensitivity. Mutant strains JSY3308, JSY3311, JSY3313, and JSY3315 (Table 1) were tested at least three times each. The five remaining MDM20 DMT strains were analyzed in less detail but demonstrated similar effects.

Computer analysis of suppressor mutations:
Coordinate data and additional instructions for reconstructing structural models of actin monomers and polymers were obtained from the internet web site generously made available by M. Lorenz (http://ergo.mpimf-heidelberg.mpg.de/~michael/) or from the Protein Data Bank (PDB) (http://www.rcsb.org/pdb). Yeast actin monomers were visualized using the coordinate file `1YAG.pdb'. The file `mdac.pdb', containing the structural coordinates for rabbit skeletal muscle actin (LORENZ et al. 1993 ), was used to build actin helices via the program `helix.c'. This program constructed new coordinate files for actin polymers from monomer data. Coordinate files were converted into images by the application RasMac v2.6 [(c) 1994–1996, Roger Sayle; http://www.umass.edu/microbio/rasmol/]. Some additional modeling of mutant actin monomers was performed with the aid of the program `O' (JONES et al. 1991 ) using coordinates `1ATN.pdb' and `1YAG.pdb'.

To find actin homologues with amino acid (aa) variations at positions equivalent to residues L221, Q314, G308 in yeast, we made use of the online application, PatScan (http://www-unix.mcs.anl.gov/compbio/PatScan/HTML/). Short Act1p sequences (11–14 aa) encompassing the residues of interest were submitted, along with the search parameters to return all matching sequences from the Swiss-Prot database containing between zero and three mismatches. At least 187 nonredundant homologous sequences, all from actin genes, were returned for each segment of Act1p submitted. Six of 189 homologues contained an amino acid difference at L221, 1 of 187 at G308, and 54 of 187 at Q314.

	RESULTS

TOP ABSTRACT MATERIALS AND METHODS RESULTS DISCUSSION LITERATURE CITED

Isolation of DMT1 and DMT2:
In an effort to identify loci that interact genetically with yeast MDM20, we designed a selection for dominant suppressor-of-mdm20 (DMT) mutations. The strategy of seeking only dominant mutations was adopted to increase the stringency of the selection. As previously reported (HERMANN et al. 1997 ; HERMANN 1998 ), overexpression of the Tpm1 protein partially suppressed the mdm20 conditional growth defect in haploid cells. This suppression was greatly reduced in homozygous mdm20/mdm20 diploid strains (HERMANN 1998 ). To minimize the probability of isolating mutations that suppressed mdm20 by simply increasing the steady-state level of Tpm1p, we screened for dominant suppressors in mdm20/mdm20 diploids.

We isolated nine spontaneous mutations capable of suppressing the mdm20/mdm20 temperature-sensitive growth defect—three mutations in JSY1371 (mdm20/mdm20; Table 1) and six mutations in JSY1372 (mdm20-1/mdm20-1; Table 1). Subsequent genetic analysis revealed no allele-specific suppression among the new DMT mutations; all nine were able to dominantly suppress the 37° growth defect in diploids homozygous for mdm20 (Fig 1). The level of growth restored by the suppressor mutations in diploids varied from weak to nearly wild type depending on the DMT allele present (Fig 1). In addition, all of the DMT mutations suppressed temperature-sensitive growth defects of the mdm20 allele in haploid strains (Fig 2).

View larger version (80K): In this window In a new window Download PPT slide   Figure 1. DMT1 and DMT2 dominant mutations suppress the temperature-sensitive growth defect of homozygous mdm20/mdm20 diploids. From top to bottom, the strains pictured are JSY1373, JSY1371, and JSY3227–3235 (Table 1). All but JSY1373 (WT) are homozygous for the deletion allele of mdm20 (mdm20). Among the suppressor-carrying strains, only JSY3227 (DMT1-1), JSY3228 (DMT1-2), and JSY3233 (DMT2-1) were isolated directly from the original selection. Alleles DMT1-3 through DMT1-6, as well as DMT2-2 and DMT2-3, were isolated in the mdm20-1/mdm20-1 background and the corresponding mdm20/mdm20 strains were constructed through subsequent crosses. All strains were serially diluted, spotted on YPD medium, and grown for 3 days at 25° or 37°.

View larger version (60K): In this window In a new window Download PPT slide   Figure 2. DMT1 and DMT2 mutations suppress the temperature-sensitive growth defect of mdm20 haploids. From top to bottom the strains pictured are JSY999, JSY1065, JSY3237, JSY3240, JSY3242, and JSY3244 (Table 1). All strains were serially diluted, spotted on YPD medium, and grown for 3 days at 25° or 37°.

Pairwise crosses of DMT mdm20 strains and subsequent tetrad analysis revealed that the original DMT mutations segregated 2:2 and defined two linkage groups, DMT1 and DMT2 (Table 2 and Table 3). As described below, the six alleles of DMT1 and the three alleles of DMT2 correspond to new alleles of the ACT1 and TPM1 genes, respectively.

DMT1 mutations fall in the ACT1 gene:
Two observations led us to suspect that DMT1 mutations fall in ACT1. First, our attempts to clone the DMT1-4 allele using a genomic library constructed from a DMT1-4 strain were unsuccessful, despite the fact that auxotrophic markers such as TRP1 and ADE2 were readily cloned from the same library (data not shown). This observation led us to suspect that yeast cells might be adversely affected by extra copies of the gene underlying DMT1 mutations, just as they are by an increased dosage of ACT1 (cited in HUFFAKER et al. 1987 ; LIU et al. 1992 ; MAGDOLEN et al. 1993 ). Second, both DMT1 and DMT2 suppressors stabilized actin cables in mdm20 cells (see below). A recent study showed that this phenotype could result from certain mutations in the ACT1 gene. BELMONT and DRUBIN 1998 described a mutation in ACT1, V159N, which restored actin cable-like structures in mdm20 mutants (though it did not rescue the mdm20 conditional growth phenotype). To determine whether the DMT1 mutations were genetically linked to ACT1, the DMT1 strains were crossed to a strain containing a functional, HIS3-marked copy of the ACT1 gene (JSY3079, ACT1::HIS3, Table 1). The segregation of histidine prototrophy and mdm20 conditional growth suppression was then analyzed in tetrads. As summarized in Table 2, the DMT1 mutations were tightly linked to the ACT1 locus. In addition, sequence analysis revealed that all of the DMT1 alleles contained mutations in the ACT1 gene that altered the predicted amino acid sequence of actin (Table 2). On the basis of these findings, we assigned each of the DMT1 mutations a new ACT1 allele number (Table 2).

DMT2 mutations fall in the TPM1 gene:
The TPM1 gene was previously identified as a multicopy suppressor of mdm20 (HERMANN et al. 1997 ). To rule out the possibility that the DMT suppressors were regulators of TPM1 expression, we used Western blot analysis to compare the steady-state level of Tpm1p in wild-type, mdm20, and mdm20 DMT strains. The steady-state level of Tpm1p in all of the strains was similar. However, migration of Tpm1p was faster than wild type in protein extracts prepared from DMT2-1 and DMT2-2 strains and slower in extracts prepared from the DMT2-3 strain, suggesting that DMT2 lesions might fall in the TPM1 coding region (data not shown). Genetic linkage and sequence analysis of the TPM1 locus from DMT2 strains verified that DMT2 mutations are new alleles of TPM1 (Table 3). Accordingly, each DMT2 mutation has been assigned a new TPM1 allele designation (Table 3). The new ACT1 and TPM1 allele numbers will be used in all subsequent discussion of the DMT mutations (refer to Table 2 and Table 3). In addition, because we observed significant phenotypic overlap among the six ACT1 suppressor alleles and among the three TPM1 alleles, data generated with two representative alleles from each group (ACT1-202, ACT1-205 and TPM1-3, TPM1-5) are shown in the remainder of this study.

Dominant ACT1 and TPM1 alleles partially restore actin cables and rescue mitochondrial inheritance defects in mdm20 cells:
The ACT1 and TPM1 suppressor alleles restored short actin cables in mdm20 cells. In wild-type cells stained with Alexa568-conjugated phalloidin at 25°, full-length actin cables extended from the tip of the bud, through the bud neck, and across the length of the mother cell to its distal tip (Fig 3B and Fig G). In mdm20 cultures, however, only 12% of the cells contained structures that resembled actin cables (Fig 3D and Fig G). These cells typically had one or two extremely short, fluorescing rods very close to the bud neck that appeared to be rudimentary actin cables (not shown). The remaining 88% of budded mdm20 cells contained no detectable actin cables, though actin patches were still present. In contrast, when ACT1 or TPM1 suppressor alleles were introduced into mdm20 strains (e.g., mdm20 ACT1-202, Fig 3F), the percentage of cells containing actin cable-like structures increased to 80% or more, depending on the allele (Fig 3G). In the majority of these cells, the suppressor mutations did not completely restore actin cables since cables still appeared shorter and less brightly stained than those in wild-type cells. As a result, images of these cables could be obtained only by overexposing actin patches in these cells (Fig 3F). The observation that restored actin cables were shorter and less bright than wild-type cables was verified in indirect immunofluorescence experiments (data not shown).

View larger version (45K): In this window In a new window Download PPT slide   Figure 3. Short actin cables emanate from the mother-bud neck in mdm20 mutants containing ACT1 or TPM1 suppressors. (A and B) Wild-type (JSY999), (C and D) mdm20 (JSY3094), and (E and F) mdm20 ACT1-202 (JSY3237) cells were grown at 25°, stained with Alexa568 phalloidin and visualized by differential interference contrast (DIC) or fluorescence microscopy. Representative cells are shown. Bar, 2 µm. (G) Quantification of actin cable restoration. Wild-type (JSY999) and mutant cells carrying mdm20 alone (JSY3094) or in combination with a suppressor mutation (JSY3237, JSY3240, JSY3242, and JSY3244) were stained with Alexa568 phalloidin to visualize actin cables and patches. Budded cells were evaluated for the presence or absence of wild-type actin cables or cable-like structures. For each strain listed, the percentages represent the average of at least two separate experiments (n 225).

In similar experiments, we found that the new ACT1 mutations do not restore actin cables in tpm1 mutant cells (data not shown). These results suggest that the ACT1 alleles do not function by eliminating the need for Tpm1p in vivo.

The dominant ACT1 and TPM1 suppressor mutations also rescued the mitochondrial inheritance defects associated with mdm20 cells. In wild-type cells grown at 25° or 37°, mitochondrial membranes visualized with the green fluorescent protein (Cox4-GFP) were transported into essentially 100% of the emerging buds (Fig 4B and Fig G). In contrast, only 72% of mdm20 buds formed at 25° and 46% of mdm20 buds formed at 37° inherited GFP-labeled mitochondrial compartments (Fig 4D and Fig G). When mdm20 mutants also carried an ACT1 or TPM1 suppressor mutation (e.g., mdm20 ACT1-202, Fig 4F) the percentage of buds that inherited mitochondria increased to >95% at 25° and >90% at 37° (Fig 4G).

View larger version (46K): In this window In a new window Download PPT slide   Figure 4. Mitochondrial inheritance is restored in mdm20 cells containing ACT1 or TPM1 suppressors. GFP-labeled mitochondrial networks (pDO12; OTSUGA et al. 1998 ) were visualized in (A and B) wild-type (JSY999), (C and D) mdm20 (JSY3094), and (E and F) mdm20 ACT1-202 (JSY3237) cells grown at 25° and shifted to 37° for 2–3 hr. DIC images (A, C, and E) and mitochondrial networks (B, D, and F) of representative cells are shown. Bar, 2 µm. (G) Quantification of mitochondrial inheritance defects. Wild-type (JSY999) and mutant cells carrying mdm20 alone (JSY3094) or mdm20 in combination with an ACT1 or TPM1 suppressor mutation (JSY3237, JSY3240, JSY3242, and JSY3244) were grown at 25° (solid bars) or 37° (shaded bars). Large-budded cells were scored for the presence or absence of mitochondria in buds. For each strain listed, the percentages represent the average of at least two separate experiments (n 125).

ACT1 and TPM1 suppressor alleles cause a small increase in Lat-A resistance:
To determine whether the new suppressor mutations conferred phenotypes on their own, haploid and homozygous diploid yeast strains carrying wild-type MDM20 and either ACT1 or TPM1 suppressor mutations were subjected to a number of different assays. Previous studies indicated that yeast strains with actin cytoskeleton defects often display increased sensitivity to high osmolarity and/or extreme temperatures (NOVICK and BOTSTEIN 1985 ; HUFFAKER et al. 1987 ; CHOWDHURY et al. 1992 ; WINSOR and SCHIEBEL 1997 ). The ACT1 and TPM1 suppressor strains, however, grew as well as wild type on medium supplemented with 0.9 M NaCl and at temperatures ranging as high as 37° and as low as 13° (data not shown). Moreover, both cell morphology and mitochondrial inheritance in these strains were indistinguishable from wild type, and staining with Alexa568 phalloidin revealed no gross defects in actin organization (data not shown).

Despite the absence of visible actin organizational defects, cells carrying ACT1 or TPM1 suppressor alleles displayed a modest increase in actin cytoskeleton stability as measured by resistance to the actin-disrupting drug, Lat-A. Lat-A inhibits actin filament formation by sequestering actin monomers (COUE et al. 1987 ) and is lethal to yeast cells at high concentrations. When filter discs containing different concentrations of Lat-A are placed on a lawn of growing cells, a halo of growth inhibition (lethal concentration zone) becomes visible around each disc. The size of this halo provides a sensitive measure of a strain's resistance to the drug (AYSCOUGH et al. 1997 ). MDM20 strains carrying ACT1 or TPM1 suppressor alleles consistently produced slightly smaller halos than wild-type strains in response to equivalent concentrations of Lat-A (Fig 5A, Fig B, and Fig E) indicating that the stability of actin structures was increased slightly in these strains.

View larger version (81K): In this window In a new window Download PPT slide   Figure 5. Sensitivity to Lat-A is decreased by the new alleles of ACT1 and TPM1. Filter discs infused with increasing concentrations of the actin-disrupting drug Lat-A (clockwise from top right: 0 mM, 0.5 mM, 1 mM, and 2 mM) were applied to fresh lawns of (A) wild-type (JSY999), (B) MDM20 ACT1-202 (JSY3308), (C) mdm20 (JSY3094), and (D) mdm20 ACT1-202 (JSY3237) cells. The plates were grown at 25° for 4 days. The complete results of a typical Lat-A experiment are shown in E. Strains not pictured in A–D, but for which results are shown in E, are JSY3311, JSY3313, JSY3315, JSY3240, JSY3242, and JSY3244 (Table 1).

An increased resistance to Lat-A was the only phenotype we detected in MDM20 cells carrying the new ACT1 and TPM1 mutations. Like the other effects of these mutations, however, enhanced Lat-A resistance was more easily observed in an mdm20 mutant background. Compared to wild type (Fig 5A), an mdm20 strain (Fig 5C) had very low Lat-A resistance and exhibited a large growth inhibition halo. Resistance was clearly increased when the mdm20 mutation was paired with a suppressor allele of ACT1 (Fig 5D and Fig E) or TPM1 (Fig 5E).

	DISCUSSION

TOP ABSTRACT MATERIALS AND METHODS RESULTS DISCUSSION LITERATURE CITED

In this study, we demonstrated that suppressors of mdm20 reside in genes encoding two major structural components of the actin cytoskeleton, ACT1 and TPM1. All nine mutations confer a dominant suppressor phenotype in diploid mdm20 strains and are able to suppress mutant phenotypes of the mdm20 allele. Each of the suppressor mutations partially or fully rescues all of the mdm20 phenotypes examined, including temperature-sensitive growth, loss of actin cables, defective mitochondrial inheritance, and Lat-A sensitivity. In an MDM20 (wild-type) background, the mutations cause a small increase in Lat-A resistance. Although the suppressor mutations alter multiple amino acids in the Act1 and Tpm1 proteins, the behavior of each suppressor is similar with respect to the different phenotypes. Our analysis of the position and nature of the different mutations suggests that they all achieve their effect by modifying the same aspect of actin organization or function. Specifically, the mdm20 suppressors appear to strengthen the interaction between actin and tropomyosin.

mdm20 suppressor mutations in ACT1:
As shown in Table 2, the mdm20 suppressor mutations in actin change the identities of residues L221, Q314, and G308. When these mutations are mapped onto a three-dimensional (3-D) model of the actin monomer (Fig 6, A–C, red), they form a small cluster at the junction of subdomains three and four (KABSCH et al. 1990 ; BOTSTEIN et al. 1997 ). The position of the mutations suggests that the substituted amino acids may affect the normal interactions of actin with the filament-binding protein tropomyosin.

View larger version (84K): In this window In a new window Download PPT slide   Figure 6. The location of mdm20 suppressor mutations on structural models of actin overlaps with the predicted binding site for tropomyosin. (A) Residues mutated in suppressor alleles of ACT1 (L221, G308, and Q314) are highlighted in red on a 3-D model of yeast actin. The position of the previously reported V159N mutation is shown in blue (BELMONT and DRUBIN 1998 ). In this view of the monomer, subdomains three and four correspond to the lower left and upper left quarters of the structure, respectively (KABSCH et al. 1990 ). On the identical structure in B, the polar residues of actin predicted to contribute to tropomyosin binding (LORENZ et al. 1995 ) are highlighted in green and a bound ATP molecule is shown in yellow. (C) A space-filling model of the structure shown in B. (D) A rabbit skeletal muscle actin 10-mer (LORENZ et al. 1993 ) showing the overlap of the ACT1 suppressor mutations (red) with the predicted tropomyosin contact sites (green).

LORENZ et al. 1995 used X-ray fiber diffraction data from oriented actin-tropomyosin gels to predict the residues of actin that are likely to be important for tropomyosin binding. When the predicted tropomyosin-binding residues are highlighted on the actin monomer (Fig 6B and Fig C, green) together with the mdm20 suppressor mutations (red), the mdm20 suppressor mutations fall within the predicted tropomyosin "footprint" and directly adjacent to several of the key tropomyosin-binding amino acids. The overlap between the tropomyosin-binding site and the mdm20 suppressor mutations in actin is even more striking in a model of a short actin polymer (Fig 6D). When viewed in this manner, the tropomyosin contact points in each monomer align along the actin filament and the mdm20 suppressor mutations clearly fall within the composite tropomyosin-binding site (note that a tropomyosin molecule spans multiple actin monomers).

At the structural level, the new mutations we have identified appear to cause only minor changes within the actin monomer. The L221F substitution increases the local bulkiness of the polypeptide chain by a small degree, as do the G308C and G308V substitutions. With respect to the changes at G308, however, the loss of flexibility normally provided by glycine at that position might actually be the more significant consequence of these mutations. Based on the crystal structure of actin, the Q314R mutation causes the loss of hydrogen bonds normally shared with T318 and V327, but the structural effects of this change should again be minor. Although the mutation at Q314 is the only one that adds a charged amino acid, all three of the substituted amino acids are located on the actin surface (polymer as well as monomer). This finding is consistent with the idea that the mutated residues exert their effect by altering the way actin interacts with one or more of its protein-binding partners.

Residues L221, G308, and Q314, like most in actin, are very highly conserved. In a survey of 187 actin homologues from a wide range of organisms (see MATERIALS AND METHODS), L221 had only six variants, all unique, and G308 had only one. Residue Q314 was changed in many homologues, but always to N, S, or T—never to R as we found in this study. In contrast, some of the variants at L221 were quite similar to the mutation we isolated at this position, L221F. Interestingly, two of the six L221 variants that were most similar came from the thermophilic fungus Thermomyces lanuginosus (F221; WILDEMAN 1988 ) and Tetrahymena thermophila (I221; CUPPLES and PEARLMAN 1986 ), organisms that thrive at temperatures up to 50° and 40°, respectively. Given that the L221F mutation allows budding yeast to overcome a temperature-sensitive growth defect, these observations raise the possibility that specific amino acid changes at residue L221 may be a recurring cellular strategy for increasing actin cytoskeleton stability at elevated temperatures.

One other actin mutation has been examined for its effect on mdm20 mutant phenotypes. BELMONT and DRUBIN 1998 showed that the V159N mutation in Act1p partially restores actin cables in mdm20 cells, though it fails to rescue the mdm20 temperature-sensitive growth defect. Cells containing the V159N mutation alone exhibit a variety of mutant actin phenotypes including slow growth at 25° and 37°, increased osmotic sensitivity, increased Lat-A resistance, and budding defects (BELMONT and DRUBIN 1998 ). In contrast, the ACT1 and TPM1 suppressor mutations described in this report do not exhibit defects associated with disrupted actin function, with the exception of increased Lat-A resistance (Fig 5). Unlike the mdm20 suppressors we isolated in subdomains three and four, the V159N mutation is located in the nucleotide binding cleft of the actin monomer (Fig 6A and Fig B) and has its primary effect on subdomain two (BELMONT et al. 1999 ). Biochemical studies indicate that V159N retards actin filament depolymerization by preventing the actin conformational change that normally occurs after Pi release (BELMONT et al. 1999 ). As a result, actin filament stability is increased in cells expressing V159N actin and these cells contain more robust actin cables and larger actin patches than normal. Although we have not yet performed biochemical studies, the phenotypes induced by our mdm20 suppressor mutations and their positions on the actin monomer suggest that they do not increase filament stability by the same mechanism as V159N. One possible explanation for the different phenotypes induced by V159N compared to the ACT1 and TPM1 suppressor mutations is that the latter mutations potentially affect actin-tropomyosin interactions exclusively. Since tropomyosin is strictly localized to actin cables (LIU and BRETSCHER 1989 ), the actin-stabilizing effect of these mutations may be cable specific. In contrast, the V159N mutation affects actin dynamics in patches as well as cables (BELMONT and DRUBIN 1998 ).

mdm20 suppressor mutations in TPM1:
Tropomyosins are rod-shaped, dimeric coiled-coil proteins (HITCHCOCK-DEGREGORI 1994 ). They align end to end along the -helical groove of actin filaments, each dimer spanning an integral number of actin monomers. Vertebrate muscle tropomyosin spans seven monomers while nonmuscle isoforms span six (MCLACHLAN and STEWART 1975 ; COTE 1983 ; PHILLIPS et al. 1986 ). Yeast Tpm1p spans only five actin monomers (LIU and BRETSCHER 1989 ; DREES et al. 1995 ). Shorter isoforms are truncated at their N termini (DREES et al. 1995 ).

Since the 3-D form of a tropomyosin monomer is basically one continuous -helix, there are few readily distinguishable structural domains (GREENFIELD and HITCHCOCK-DEGREGORI 1995 ). However, the comparison of different tropomyosin isoforms, particularly from vertebrates, has revealed that distinct regions of the protein are variable while others are more highly conserved. Most differences between vertebrate tropomyosin isoforms occur at the N or C termini or at a specific central region defined by a variable exon (exon VI; LEES-MILLER and HELFMAN 1991 ; LIN et al. 1997 ). These three variable regions appear to be responsible for isoform-specific functions. The mdm20 suppressor mutations we identified in TPM1 encode changes at the N terminus and at residue S112 (Table 3). According to published alignments, S112 in yeast aligns with the central variable region of vertebrate tropomyosin isoforms (DREES et al. 1995 ; LIN et al. 1997 ). Thus, each of our TPM1 suppressor mutations appears to fall within a region regularly exploited by evolution to modulate tropomyosin function.

The fact that we isolated two independent mutations at S112 suggests that this residue plays an important role in Tpm1p function. One obvious possibility is that S112 is phosphorylated. Several observations support the idea that S112 may be a target for phosphorylation. First, in the two closest Tpm1p homologues, S. cerevisiae Tpm2p and Schizosaccharomyces pombe Cdc8p, the positions analogous to S112 contain an S and an E residue, respectively (BALASUBRAMANIAN et al. 1992 ; DREES et al. 1995 ). The negative charge on the glutamate side chain of S. pombe tropomyosin could be introduced in S. cerevisiae isoforms by the addition of a phosphate group to the corresponding serine residue. Second, several vertebrate tropomyosins are phosphorylated (MAK et al. 1978 ; MONTARRAS et al. 1981 ; HEELEY et al. 1982 ; EDWARDS and ROMERO-HERRERA 1983 ), albeit at the C terminus rather than the central variable region (MAK et al. 1978 ). A vertebrate tropomyosin kinase has been identified but not well characterized (MONTGOMERY and MAK 1984 ; DEBELLE and MAK 1987 ). Phosphorylation at the C termini of vertebrate tropomyosins is thought to be a mechanism by which troponin (in muscle cells; HEELEY et al. 1989 ) and perhaps caldesmon (in nonmuscle cells) regulate tropomyosin-actin interactions. Although database searches of the S. cerevisiae genome reveal no clear homologues of troponin or caldesmon, it is possible that phosphorylation of S112 modulates the interaction of Tpm1p with actin via an analogous regulatory protein.

An alternative possibility is that phosphorylation of S112 normally influences the stability or conformation of the Tpm1p dimer. Coiled-coil proteins like tropomyosin are defined by the pseudoheptapeptide (heptad) repeat motif (COHEN and PARRY 1986 ). Within each heptad, residues at the different positions, designated a–g, have typical characteristics. Positions a and d are normally filled by hydrophobic residues that form the core of the coiled-coil structure. In contrast, residues at positions b, c, e, f, and g are hydrophilic and solvent exposed in the dimerized protein. In the heptad repeat scheme of Tpm1p, S112 is located at an a position (LIU and BRETSCHER 1989 ), placing it at the interface of the Tpm1p dimer and suggesting that the primary effect of S112 phosphorylation could be to block standard coiled-coil interactions in this region of the protein. According to this scenario, the S112F and S112Y mutations we have isolated would eliminate Tpm1p phosphorylation and potentially increase dimer stability or induce a conformational change (GREENFIELD and HITCHCOCK-DEGREGORI 1995 ). Either result could improve binding of the Tpm1p dimer to actin filaments, ultimately increasing the stability of actin filaments and cables. We are currently examining the possibility that Tpm1p residue S112 is phosphorylated in vivo.

The TPM1-5 mutation adds seven amino acids (MHTKKAT) to the N terminus of Tpm1p (Table 3). These additional amino acids might affect the end-to-end polymerization of Tpm1p dimers and/or the affinity of Tpm1p for the actin filament (HITCHCOCK-DEGREGORI 1994 ). The N-terminal extension might improve actin binding by mimicking N-terminal acetylation. Although it is not known whether yeast Tpm1p is acetylated at its N terminus, this modification is critical for strong actin binding in vertebrate muscle tropomyosin (HITCHCOCK-DEGREGORI and HEALD 1987 ; URBANCIKOVA and HITCHCOCK-DEGREGORI 1994 ). The same high level of actin binding can be induced in bacterially expressed, unacetylated tropomyosin by the addition of a few extra amino acids at the N terminus (MONTEIRO et al. 1994 ; URBANCIKOVA and HITCHCOCK-DEGREGORI 1994 ). The composition of the fusion peptide appears to be unimportant and small peptides (11 aa) do not interfere with tropomyosin polymerization (URBANCIKOVA and HITCHCOCK-DEGREGORI 1994 ). On the basis of these data, it seems possible that the addition of seven N-terminal amino acids might increase the actin-binding activity of Tpm1p and the stability of actin filaments.

Mechanism of mdm20 suppression:
The simplest explanation for our findings is that the ACT1 and TPM1 mutations we have isolated suppress mdm20 defects by slightly adjusting normal actin-tropomyosin interactions such that actin filament and cable stability is enhanced. Since the ACT1 and TPM1 mutations are able to suppress mutant phenotypes in mdm20 null strains, they can clearly bypass the requirement for MDM20 function in yeast. However, our data leave open the possibility that Mdm20p regulates interactions between the actin and tropomyosin proteins. In the future, biochemical assays can be used to study the actin assembly/turnover properties and actin filament-binding properties of the purified mutant actin and tropomyosin proteins described here. Additional biochemical studies using purified Mdm20p are necessary to determine whether this protein contributes primarily to actin-tropomyosin interactions or another aspect of actin filament dynamics.

	FOOTNOTES

¹ Present address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., A3-013, Seattle, WA 98109-1024.

	ACKNOWLEDGMENTS

We are grateful to Lisa Belmont, David Drubin, and Anthony Bretscher for strains, mutants, and plasmids and to Tatiana Karpova and John Cooper for goat anti-actin antibody. We especially thank Polycarpe Songfack for assistance with computer graphics and Jon Seger and Josh Cherry for sharing their statistical expertise. We also thank Anthony Bretscher, Michael Mathews, Denichiro Otsuga, Frank Whitby, and members of the Shaw laboratory for helpful discussions and careful review of the manuscript. This work was supported by grant GM-53466 from the National Institutes of Health (NIH) to J. Shaw. J. Singer was supported in part by a Huntsman Cancer Institute Graduate Fellowship and an NIH Predoctoral Genetics training grant (T32-GM07464). G. Hermann received support from an NIH Predoctoral Genetics training grant (T32-GM07464) and a University of Utah Graduate Research Fellowship.

Note added in proof: Since the initial submission of this manuscript, we have identified two additional ACT1 alleles and one additional TPM1 allele capable of suppressing mdm20. Preliminary analyses suggest that these mutations behave similarly to those described in this study. Indeed, two of the three are identical to previously identified mutations: = G308C; = S112Y. The remaining mutation is novel: = L185F.

Manuscript received March 20, 2000; Accepted for publication June 9, 2000.

	LITERATURE CITED

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