GENETIC ANALYSIS OF MATING TYPE DIFFERENTIATION IN PARAMECIUM TETRAURELIA. II. ROLE OF THE MICRONUCLEI IN MATING-TYPE DETERMINATION

Y. Brygoo ¹, T. M. Sonneborn ², A. M. Keller ¹, R. V. Dippell ², and M. V. Schneller ²

¹ Centre de Génétique Moléculaire du C.N.R.S. 91190 Gif-sur-Yvette, France
² Department of Biology, Indiana University, Bloomington, Indiana 47401

The two complementary mating types, O and E, of Paramecium tetraurelia are normally inherited cytoplasmically. This property has generally been interpreted to indicate the presence of cytoplasmic factors that determine macronuclear differentiation towards O or E. In these macronuclear-cytoplasmic interactions, the micronuclei were held to be unbiased and the determination to be established in the course of macronuclear development. In order to ascertain whether the micronuclei were actually neutral, amicronucleate clones were needed and a method to produce them was developed. In crosses between amicronucleate clones and normal micronucleate clones, we have observed regular deviations from cytoplasmic inheritance: the commonest deviation is that most O amicronucleate cells become E when they receive a micronucleus from an E partner. The data can be interpreted by assuming that the micronuclei are predetermined and that the apparent "cytoplasmic" inheritance of the two mating types is due, in E cells, to E-determining factors present in the cytoplasm and in the nucleus; and, in O cells, to O-determining factors present only or mainly in the nucleus.

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