- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Raju, N. B.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Raju, N. B.
CYTOGENETIC BEHAVIOR OF SPORE KILLER GENES IN NEUROSPORA
Namboori B. Raju 1
1 Department of Biological Sciences, Stanford University, Stanford, California 94305
Crosses heterozygous and homozygous for Sk-1, Sk-2 and Sk-3 were examined by light microscopy. All three Spore killers behave similarly. In heterozygous killer x sensitive crosses, meiosis and ascospore development are normal until after the second postmeiotic mitosis when four of the eight ascospores in each ascus stop developing and degenerate. The four surviving ascospores carry the killer. Death of sensitives thus occurs only after killer and sensitive alleles, SkK and SkS, have segregated into separate ascospores. Homozygous killer x killer crosses do not show such a pattern of degeneration. Either all ascospores are normal or, if some fail to mature, they do not resemble the degenerating sensitive ascospores in heterozygous asci.——With Sk-2, it was shown that SkS nuclei do not abort when both SkK and SkS are present in the same ascospore. Mutants affecting ascus development were used to obtain large ascospores enclosing both SkK and SkS meiotic products in a common cytoplasm. SkS nuclei do not then undergo the degeneration that would be seen if they were sequestered into separate ascospores, and viable SkS progeny are recovered in undiminished numbers when the mixed multinucleate large ascospores are germinated. In a four-spored mutant, where each ascospore encloses a single nucleus following meiosis, degeneration of SkS ascospores nevertheless occurs, even though the third nuclear division is omitted. Cycloheximide and temperature treatments do not affect the expression of SkK.
Submitted on March 5, 1979Revised on May 21, 1979
This article has been cited by other articles:
 |
|
 |
|
  N. B. Raju, R. L. Metzenberg, and P. K. T. Shiu Neurospora Spore Killers Sk-2 and Sk-3 Suppress Meiotic Silencing by Unpaired DNA Genetics, May 1, 2007; 176(1): 43 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. P. Dalstra, K. Swart, A. J. M. Debets, S. J. Saupe, and R. F. Hoekstra Sexual transmission of the [Het-s] prion leads to meiotic drive in Podospora anserina PNAS, May 27, 2003; 100(11): 6616 - 6621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. van der Gaag, A. J. M. Debets, J. Oosterhof, M. Slakhorst, J. A. G. M. Thijssen, and R. F. Hoekstra Spore-Killing Meiotic Drive Factors in a Natural Population of the Fungus Podospora anserina Genetics, October 1, 2000; 156(2): 593 - 605. [Abstract] [Full Text]
|
|