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- Articles by Staelens, J.
- Articles by Vuylsteke, M.
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- Articles by Staelens, J.
- Articles by Vuylsteke, M.
Genetics, Vol. 179, 917-925, June 2008, Copyright © 2008
doi:10.1534/genetics.107.080150
High-Density Linkage Maps and Sex-Linked Markers for the Black Tiger Shrimp (Penaeus monodon)
Jan Staelens*,
,
,
Debbie Rombaut*,,
Ilse Vercauteren*,,
Brad Argue,
John Benzie and
Marnik Vuylsteke*,,1
* Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium, Department of Molecular Genetics, Ghent University, B-9052 Ghent, Belgium and Moana Technologies, Kailua Kona, Hawaii 96740
1 Corresponding author: Department of Plant Systems Biology, VIB, Technologiepark 927, B-9052 Ghent, Belgium.
E-mail: marnik.vuylsteke{at}psb.ugent.be
We report on the construction of sex-specific high-density linkage maps and identification of sex-linked markers for the black tiger shrimp (Penaeus monodon). Overall, we identified 44 male and 43 female linkage groups (2n = 88) from the analysis of 2306 AFLP markers segregating in three full-sib families, covering 2378 and 2362 cM, respectively. Twenty-one putatively homologous linkage groups, including the sex-linkage groups, were identified between the female and male linkage maps. Six sex-linked AFLP marker alleles were inherited from female parents in the three families, suggesting that the P. monodon adopts a WZ–ZZ sex-determining system. Two sex-linked AFLP markers, one of which we converted into an allele-specific assay, confirmed their association with sex in a panel of 52 genetically unrelated animals.