- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Data Supplement
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Chamberlain, K. L.
- Articles by Keller, L. R.
- Search for Related Content
- PUBMED
- Articles by Chamberlain, K. L.
- Articles by Keller, L. R.
Genetics, Vol. 179, 7-19, May 2008, Copyright © 2008
doi:10.1534/genetics.107.082149
Gene Expression Profiling of Flagellar Disassembly in Chlamydomonas reinhardtii
Kara L. Chamberlain, Steven H. Miller and Laura R. Keller1
Department of Biological Science, Florida State University, Tallahassee, Florida 32306-4370
1 Corresponding author: Department of Biological Science, Florida State University, Tallahassee, FL 32306-4370.
E-mail: lkeller{at}bio.fsu.edu
Flagella are sensory organelles that interact with the environment through signal transduction and gene expression networks. We used microarray profiling to examine gene regulation associated with flagellar length change in the green alga Chlamydomonas reinhardtii. Microarrays were probed with fluorescently labeled cDNAs synthesized from RNA extracted from cells before and during flagellar assembly or disassembly. Evaluation of the gene expression profiles identified >100 clones showing at least a twofold change in expression during flagellar length changes. Products of these genes are associated not only with flagellar structure and motility but also with other cellular responses, including signal transduction and metabolism. Expression of specific genes from each category was further characterized at higher resolution by using quantitative real-time PCR (qRT–PCR). Analysis and comparison of the gene expression profiles coupled to flagellar assembly and disassembly revealed that each process involves a new and uncharacterized whole-cell response to flagellar length changes. This analysis lays the groundwork for a more comprehensive understanding of the cellular and molecular networks regulating flagellar length changes.
This article has been cited by other articles:
 |
|
 |
|
  O. Vallon and S. Dutcher Treasure Hunting in the Chlamydomonas Genome Genetics, May 1, 2008; 179(1): 3 - 6. [Full Text] [PDF]
|
|