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Genetics, Vol. 178, 1251-1269, March 2008, Copyright © 2008
doi:10.1534/genetics.106.067603
Reduced Mismatch Repair of Heteroduplexes Reveals "Non"-interfering Crossing Over in Wild-Type Saccharomyces cerevisiae
Tony J. Getz1, Stephen A. Banse2, Lisa S. Young, Allison V. Banse2, Johanna Swanson3, Grace M. Wang4, Barclay L. Browne, Henriette M. Foss and Franklin W. Stahl5
Institute of Molecular Biology and Department of Biology, University of Oregon, Eugene, Oregon 97403-1229
5 Corresponding author: Institute of Molecular Biology, 1370 Franklin Blvd., University of Oregon, Eugene, OR 97403-1229.
E-mail: fstahl{at}uoregon.edu
Using small palindromes to monitor meiotic double-strand-break-repair (DSBr) events, we demonstrate that two distinct classes of crossovers occur during meiosis in wild-type yeast. We found that crossovers accompanying 5:3 segregation of a palindrome show no conventional (i.e., positive) interference, while crossovers with 6:2 or normal 4:4 segregation for the same palindrome, in the same cross, do manifest interference. Our observations support the concept of a "non"-interference class and an interference class of meiotic double-strand-break-repair events, each with its own rules for mismatch repair of heteroduplexes. We further show that deletion of MSH4 reduces crossover tetrads with 6:2 or normal 4:4 segregation more than it does those with 5:3 segregation, consistent with Msh4p specifically promoting formation of crossovers in the interference class. Additionally, we present evidence that an ndj1 mutation causes a shift of noncrossovers to crossovers specifically within the "non"-interference class of DSBr events. We use these and other data in support of a model in which meiotic recombination occurs in two phases—one specializing in homolog pairing, the other in disjunction—and each producing both noncrossovers and crossovers.
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