- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Schar, P.
- Articles by Kohli, J.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Schar, P.
- Articles by Kohli, J.
Genetics, Vol 133, 825-835, Copyright © 1993
INVESTIGATIONS |
Marker Effects of G to C Transversions on Intragenic Recombination and Mismatch Repair in Schizosaccharomyces pombe
P. Schar and J. Kohli
Institute of General Microbiology, University of Bern, CH-3012 Bern, Switzerland
G to C transversion mutations show very strong allele-specific marker effects on the frequency of wild-type recombinants in intragenic two-factor crosses. Here we present a detailed study of the marker effect of one representative, the ade6-M387 mutation of Schizosaccharomyces pombe. Crosses of M387 with other mutations at varying distance reveal highly increased prototroph frequencies in comparison with the C to T transition mutation ade6-51 (control without any known marker effect) located four nucleotides from M387. The marker effect of M387 is strongest (>40-fold) for crosses with mutations less than 15 nucleotides from M387. It decreases to an intermediate level (5-10-fold) in crosses with mutations located 25-150 base pairs from M387/51 and is very low in crosses with mutations beyond 200 base pairs. On the basis of these results and the quantitation of the low efficiency of C/C mismatch repair presented in the accompanying publication we propose the existence of at least two different types of mechanisms for base mismatch repair in fission yeast. The major system is suggested to recognize all base mismatches except C/C with high efficiency and to generate long excision tracts (approximately 100 nucleotides unidirectionally). The minor system is proposed to recognize all base mismatches including C/C with low and variable efficiency and to have short excision tracts (approximately 10 nucleotides unidirectionally). We estimate from the M387 marker effect that the minor system accounts for approximately 1-8% repair of non-C/C mismatches (depending on the nature of the mutation) in fission yeast meiosis.
This article has been cited by other articles:
 |
|
 |
|
  J. Gao, M. K. Davidson, and W. P. Wahls Distinct regions of ATF/CREB proteins Atf1 and Pcr1 control recombination hotspot ade6-M26 and the osmotic stress response Nucleic Acids Res., May 1, 2008; 36(9): 2838 - 2851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Baur, E. Hartsuiker, E. Lehmann, K. Ludin, P. Munz, and J. Kohli The Meiotic Recombination Hot Spot ura4A in Schizosaccharomyces pombe Genetics, February 1, 2005; 169(2): 551 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Muheim-Lenz, T. Buterin, G. Marra, and H. Naegeli Short-patch correction of C/C mismatches in human cells Nucleic Acids Res., December 21, 2004; 32(22): 6696 - 6705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Marti, C. Kunz, and O. Fleck Repair of Damaged and Mismatched DNA by the XPC Homologues Rhp41 and Rhp42 of Fission Yeast Genetics, June 1, 2003; 164(2): 457 - 467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Tornier, S. Bessone, I. Varlet, C. Rudolph, M. Darmon, and O. Fleck Requirement for Msh6, but Not for Swi4 (Msh3), in Msh2-Dependent Repair of Base-Base Mismatches and Mononucleotide Loops in Schizosaccharomyces pombe Genetics, May 1, 2001; 158(1): 65 - 75. [Abstract] [Full Text]
|
|
|
|
|
|
T. Nakahara, Q.-M. Zhang, K. Hashiguchi, and S. Yonei Identification of proteins of Escherichia coli and Saccharomyces cerevisiae that specifically bind to C/C mismatches in DNA Nucleic Acids Res., July 1, 2000; 28(13): 2551 - 2556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kaur, J. L. A. Fraser, G. A. Freyer, S. Davey, and P. W. Doetsch A Uve1p-Mediated Mismatch Repair Pathway in Schizosaccharomyces pombe Mol. Cell. Biol., July 1, 1999; 19(7): 4703 - 4710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Rudolph, C. Kunz, S. Parisi, E. Lehmann, E. Hartsuiker, B. Fartmann, W. Kramer, J. Kohli, and O. Fleck The msh2 Gene of Schizosaccharomyces pombe Is Involved in Mismatch Repair, Mating-Type Switching, and Meiotic Chromosome Organization Mol. Cell. Biol., January 1, 1999; 19(1): 241 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Fleck, C. Kunz, C. Rudolph, and J. Kohli The High Mobility Group Domain Protein Cmb1 of Schizosaccharomyces pombe Binds to Cytosines in Base Mismatches and Opposite Chemically Altered Guanines J. Biol. Chem., November 13, 1998; 273(46): 30398 - 30405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Taghian, H. Hough, and J. A. Nickoloff Biased Short Tract Repair of Palindromic Loop Mismatches in Mammalian Cells Genetics, March 1, 1998; 148(3): 1257 - 1268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Evidence for Independent Mismatch Repair Processing on Opposite Sides of a Double-Strand Break in Saccharomyces cerevisiae Genetics, January 1, 1998; 148(1): 59 - 70.
|
|
|
|
 |
|
 P Szankasi and G. Smith A role for exonuclease I from S. pombe in mutation avoidance and mismatch correction Science, February 24, 1995; 267(5201): 1166 - 1169. [Abstract] [PDF]
|
|
|
|
|
|
M. Hohl, O. Christensen, C. Kunz, H. Naegeli, and O. Fleck Binding and Repair of Mismatched DNA Mediated by Rhp14, the Fission Yeast Homologue of Human XPA J. Biol. Chem., August 10, 2001; 276(33): 30766 - 30772. [Abstract] [Full Text] [PDF]
|
|