The Genetic Control of Direct-Repeat Recombination in Saccharomyces: The Effect of rad52 and rad1 on Mitotic Recombination at GAL10, a Transcriptionally Regulated Gene

INVESTIGATIONS

The Genetic Control of Direct-Repeat Recombination in Saccharomyces: The Effect of rad52 and rad1 on Mitotic Recombination at GAL10, a Transcriptionally Regulated Gene

B. J. Thomas and R. Rothstein
Current address: Department of Biological Chemistry, University of California, Los Angeles, California 90024.

We have previously shown direct-repeat recombination events leading to loss of a plasmid integrated at the GAL10 locus in Saccharomyces cerevisiae are stimulated by transcription of the region. We have examined the role of two recombination- and repair-defective mutations, rad1 and rad52, on direct repeat recombination in transcriptionally active and inactive sequences. We show that the RAD52 gene is required for transcription-stimulated recombination events leading to loss of the integrated plasmid. Similarly, Gal(+) events between the duplicated repeats that retain the integrated plasmid DNA (Gal(+) Ura(+) replacement events) are reduced 20-fold in the rad52 mutant in sequences that are constitutively expressed. In contrast, in sequences that are not expressed, the rad52 mutation reduces plasmid loss events by only twofold and Gal(+) Ura(+) replacements by fourfold. We also observe an increase in disome-associated plasmid loss events in the rad52 mutant, indicative of chromosome gain. This event is not affected by expression of the region. Plasmid loss events in rad1 mutant strains are reduced only twofold in transcriptionally active sequences and are not affected in sequences that are repressed. However, the rad1 and rad52 double mutant shows a decrease in plasmid loss events greater than the sum of the decreases in the rates of this event displayed by either single mutant in both constitutive and repressed DNA, indicating a synergistic interaction between these two genes. The synergism is limited to recombination since the rad1 rad52 double mutant is no more sensitive when compared with either single mutant in its ability to survive radiation damage. Finally, the recombination pathway that remains in the double mutant is positively affected by transcription of the region.

This article has been cited by other articles:

W. G. Waples, C. Chahwan, M. Ciechonska, and B. D. Lavoie
Putting the Brake on FEAR: Tof2 Promotes the Biphasic Release of Cdc14 Phosphatase during Mitotic Exit
Mol. Biol. Cell, January 1, 2009; 20(1): 245 - 255.
[Abstract] [Full Text] [PDF]

E. Mito, J. V. Mokhnatkin, M. C. Steele, V. L. Buettner, S. S. Sommer, G. M. Manthey, and A. M. Bailis
Mutagenic and Recombinagenic Responses to Defective DNA Polymerase {delta} Are Facilitated by the Rev1 Protein in pol3-t Mutants of Saccharomyces cerevisiae
Genetics, August 1, 2008; 179(4): 1795 - 1806.
[Abstract] [Full Text] [PDF]

S. Keppler-Ross, C. Noffz, and N. Dean
A New Purple Fluorescent Color Marker for Genetic Studies in Saccharomyces cerevisiae and Candida albicans
Genetics, May 1, 2008; 179(1): 705 - 710.
[Abstract] [Full Text] [PDF]

N. Averbeck, X.-D. Gao, S.-I. Nishimura, and N. Dean
Alg13p, the Catalytic Subunit of the Endoplasmic Reticulum UDP-GlcNAc Glycosyltransferase, Is a Target for Proteasomal Degradation
Mol. Biol. Cell, May 1, 2008; 19(5): 2169 - 2178.
[Abstract] [Full Text] [PDF]

L. Maloisel, F. Fabre, and S. Gangloff
DNA Polymerase {delta} Is Preferentially Recruited during Homologous Recombination To Promote Heteroduplex DNA Extension
Mol. Cell. Biol., February 15, 2008; 28(4): 1373 - 1382.
[Abstract] [Full Text] [PDF]

A. Montanari, C. Besagni, C. De Luca, V. Morea, R. Oliva, A. Tramontano, M. Bolotin-Fukuhara, L. Frontali, and S. Francisci
Yeast as a model of human mitochondrial tRNA base substitutions: Investigation of the molecular basis of respiratory defects
RNA, February 1, 2008; 14(2): 275 - 283.
[Abstract] [Full Text] [PDF]

S. Flott, C. Alabert, G. W. Toh, R. Toth, N. Sugawara, D. G. Campbell, J. E. Haber, P. Pasero, and J. Rouse
Phosphorylation of Slx4 by Mec1 and Tel1 Regulates the Single-Strand Annealing Mode of DNA Repair in Budding Yeast
Mol. Cell. Biol., September 15, 2007; 27(18): 6433 - 6445.
[Abstract] [Full Text] [PDF]

M. S. Navarro, L. Bi, and A. M. Bailis
A Mutant Allele of the Transcription Factor IIH Helicase Gene, RAD3, Promotes Loss of Heterozygosity in Response to a DNA Replication Defect in Saccharomyces cerevisiae
Genetics, July 1, 2007; 176(3): 1391 - 1402.
[Abstract] [Full Text] [PDF]

M. A. Barbera and T. D. Petes
Selection and analysis of spontaneous reciprocal mitotic cross-overs in Saccharomyces cerevisiae
PNAS, August 22, 2006; 103(34): 12819 - 12824.
[Abstract] [Full Text] [PDF]

K. C. Lindstrom, J. C. Vary Jr., M. R. Parthun, J. Delrow, and T. Tsukiyama
Isw1 Functions in Parallel with the NuA4 and Swr1 Complexes in Stress-Induced Gene Repression.
Mol. Cell. Biol., August 1, 2006; 26(16): 6117 - 6129.
[Abstract] [Full Text] [PDF]

A. Palomino, P. Herrero, and F. Moreno
Tpk3 and Snf1 protein kinases regulate Rgt1 association with Saccharomyces cerevisiae HXK2 promoter
Nucleic Acids Res., March 9, 2006; 34(5): 1427 - 1438.
[Abstract] [Full Text] [PDF]

B. O. Krogh, B. Llorente, A. Lam, and L. S. Symington
Mutations in Mre11 Phosphoesterase Motif I That Impair Saccharomyces cerevisiae Mre11-Rad50-Xrs2 Complex Stability in Addition to Nuclease Activity
Genetics, December 1, 2005; 171(4): 1561 - 1570.
[Abstract] [Full Text] [PDF]

X.-D. Gao, H. Tachikawa, T. Sato, Y. Jigami, and N. Dean
Alg14 Recruits Alg13 to the Cytoplasmic Face of the Endoplasmic Reticulum to Form a Novel Bipartite UDP-N-acetylglucosamine Transferase Required for the Second Step of N-Linked Glycosylation
J. Biol. Chem., October 28, 2005; 280(43): 36254 - 36262.
[Abstract] [Full Text] [PDF]

S. Huang, H. Zhou, D. Katzmann, M. Hochstrasser, E. Atanasova, and Z. Zhang
Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing
PNAS, September 20, 2005; 102(38): 13410 - 13415.
[Abstract] [Full Text] [PDF]

L. D. Langston and L. S. Symington
Gene targeting in yeast is initiated by two independent strand invasions
PNAS, October 26, 2004; 101(43): 15392 - 15397.
[Abstract] [Full Text] [PDF]

S. Nicholls, M. Straffon, B. Enjalbert, A. Nantel, S. Macaskill, M. Whiteway, and A. J. P. Brown
Msn2- and Msn4-Like Transcription Factors Play No Obvious Roles in the Stress Responses of the Fungal Pathogen Candida albicans
Eukaryot. Cell, October 1, 2004; 3(5): 1111 - 1123.
[Abstract] [Full Text] [PDF]

L. Krejci, M. Macris, Y. Li, S. Van Komen, J. Villemain, T. Ellenberger, H. Klein, and P. Sung
Role of ATP Hydrolysis in the Antirecombinase Function of Saccharomyces cerevisiae Srs2 Protein
J. Biol. Chem., May 28, 2004; 279(22): 23193 - 23199.
[Abstract] [Full Text] [PDF]

S. Grossi, A. Puglisi, P. V. Dmitriev, M. Lopes, and D. Shore
Pol12, the B subunit of DNA polymerase {alpha}, functions in both telomere capping and length regulation
Genes & Dev., May 1, 2004; 18(9): 992 - 1006.
[Abstract] [Full Text] [PDF]

T. A. Motycka, T. Bessho, S. M. Post, P. Sung, and A. E. Tomkinson
Physical and Functional Interaction between the XPF/ERCC1 Endonuclease and hRad52
J. Biol. Chem., April 2, 2004; 279(14): 13634 - 13639.
[Abstract] [Full Text] [PDF]

T. Uchiki, L. T. Dice, R. L. Hettich, and C. Dealwis
Identification of Phosphorylation Sites on the Yeast Ribonucleotide Reductase Inhibitor Sml1
J. Biol. Chem., March 19, 2004; 279(12): 11293 - 11303.
[Abstract] [Full Text] [PDF]

A. P. Davis and L. S. Symington
RAD51-Dependent Break-Induced Replication in Yeast
Mol. Cell. Biol., March 15, 2004; 24(6): 2344 - 2351.
[Abstract] [Full Text] [PDF]

J.-L. Ma, E. M. Kim, J. E. Haber, and S. E. Lee
Yeast Mre11 and Rad1 Proteins Define a Ku-Independent Mechanism To Repair Double-Strand Breaks Lacking Overlapping End Sequences
Mol. Cell. Biol., December 1, 2003; 23(23): 8820 - 8828.
[Abstract] [Full Text] [PDF]

P. A. Mieczkowski, J. O. Mieczkowska, M. Dominska, and T. D. Petes
Genetic regulation of telomere-telomere fusions in the yeast Saccharomyces cerevisae
PNAS, September 16, 2003; 100(19): 10854 - 10859.
[Abstract] [Full Text] [PDF]

A. Galli, T. Cervelli, and R. H. Schiestl
Characterization of the Hyperrecombination Phenotype of the pol3-t Mutation of Saccharomyces cerevisiae
Genetics, May 1, 2003; 164(1): 65 - 79.
[Abstract] [Full Text] [PDF]

M. M. Kessler, Q. Zeng, S. Hogan, R. Cook, A. J. Morales, and G. Cottarel
Systematic Discovery of New Genes in the Saccharomyces cerevisiae Genome
Genome Res., February 1, 2003; 13(2): 264 - 271.
[Abstract] [Full Text] [PDF]

J. C. Vary Jr., V. K. Gangaraju, J. Qin, C. C. Landel, C. Kooperberg, B. Bartholomew, and T. Tsukiyama
Yeast Isw1p Forms Two Separable Complexes In Vivo
Mol. Cell. Biol., January 1, 2003; 23(1): 80 - 91.
[Abstract] [Full Text]

L. S. Symington
Role of RAD52 Epistasis Group Genes in Homologous Recombination and Double-Strand Break Repair
Microbiol. Mol. Biol. Rev., December 1, 2002; 66(4): 630 - 670.
[Abstract] [Full Text] [PDF]

J. A. Freedman and S. Jinks-Robertson
Genetic Requirements for Spontaneous and Transcription-Stimulated Mitotic Recombination in Saccharomyces cerevisiae
Genetics, September 1, 2002; 162(1): 15 - 27.
[Abstract] [Full Text] [PDF]

G. M. Manthey and A. M. Bailis
Multiple Pathways Promote Short-Sequence Recombination in Saccharomyces cerevisiae
Mol. Cell. Biol., August 1, 2002; 22(15): 5347 - 5356.
[Abstract] [Full Text] [PDF]

M. C. V. L. Wong, S. R. S. Scott-Drew, M. J. Hayes, P. J. Howard, and J. A. H. Murray
RSC2, Encoding a Component of the RSC Nucleosome Remodeling Complex, Is Essential for 2{micro}m Plasmid Maintenance in Saccharomyces cerevisiae
Mol. Cell. Biol., June 15, 2002; 22(12): 4218 - 4229.
[Abstract] [Full Text] [PDF]

U. H. Mortensen, N. Erdeniz, Q. Feng, and R. Rothstein
A Molecular Genetic Dissection of the Evolutionarily Conserved N Terminus of Yeast Rad52
Genetics, June 1, 2002; 161(2): 549 - 562.
[Abstract] [Full Text] [PDF]

S. Grossi, A. Bianchi, P. Damay, and D. Shore
Telomere Formation by Rap1p Binding Site Arrays Reveals End-Specific Length Regulation Requirements and Active Telomeric Recombination
Mol. Cell. Biol., December 1, 2001; 21(23): 8117 - 8128.
[Abstract] [Full Text] [PDF]

S. Moreau, E. A. Morgan, and L. S. Symington
Overlapping Functions of the Saccharomyces cerevisiae Mre11, Exo1 and Rad27 Nucleases in DNA Metabolism
Genetics, December 1, 2001; 159(4): 1423 - 1433.
[Abstract] [Full Text] [PDF]

A. P. Davis and L. S. Symington
The Yeast Recombinational Repair Protein Rad59 Interacts With Rad52 and Stimulates Single-Strand Annealing
Genetics, October 1, 2001; 159(2): 515 - 525.
[Abstract] [Full Text] [PDF]

T. Hishida, H. Iwasaki, T. Ohno, T. Morishita, and H. Shinagawa
A yeast gene, MGS1, encoding a DNA-dependent AAA+ ATPase is required to maintain genome stability
PNAS, July 17, 2001; 98(15): 8283 - 8289.
[Abstract] [Full Text] [PDF]

E. Perkins, D. Sun, A. Nguyen, S. Tulac, M. Francesco, H. Tavana, H. Nguyen, S. Tugendreich, P. Barthmaier, J. Couto, et al.
Novel Inhibitors of Poly(ADP-ribose) Polymerase/PARP1 and PARP2 Identified Using a Cell-based Screen in Yeast
Cancer Res., May 1, 2001; 61(10): 4175 - 4183.
[Abstract] [Full Text]

M. C. Negritto, J. Qiu, D. O. Ratay, B. Shen, and A. M. Bailis
Novel Function of Rad27 (FEN-1) in Restricting Short-Sequence Recombination
Mol. Cell. Biol., April 1, 2001; 21(7): 2349 - 2358.
[Abstract] [Full Text]

L. Krejci, J. Damborsky, B. Thomsen, M. Duno, and C. Bendixen
Molecular Dissection of Interactions between Rad51 and Members of the Recombination-Repair Group
Mol. Cell. Biol., February 1, 2001; 21(3): 966 - 976.
[Abstract] [Full Text]

A. K. Pearce, K. Crimmins, E. Groussac, M. J. E. Hewlins, J. R. Dickinson, J. Francois, I. R. Booth, and A. J. P. Brown
Pyruvate kinase (Pyk1) levels influence both the rate and direction of carbon flux in yeast under fermentative conditions
Microbiology, February 1, 2001; 147(2): 391 - 401.
[Abstract] [Full Text]

A. K. Pearce, I. R. Booth, and A. J. P. Brown
Genetic manipulation of 6-phosphofructo-1-kinase and fructose 2,6-bisphosphate levels affects the extent to which benzoic acid inhibits the growth of Saccharomyces cerevisiae
Microbiology, February 1, 2001; 147(2): 403 - 410.
[Abstract] [Full Text]

H. L. Klein
Mutations in Recombinational Repair and in Checkpoint Control Genes Suppress the Lethal Combination of srs2{{Delta}} With Other DNA Repair Genes in Saccharomyces cerevisiae
Genetics, February 1, 2001; 157(2): 557 - 565.
[Abstract] [Full Text]

L. S. Symington, L. E. Kang, and S. Moreau
Alteration of gene conversion tract length and associated crossing over during plasmid gap repair in nuclease-deficient strains of Saccharomyces cerevisiae
Nucleic Acids Res., December 1, 2000; 28(23): 4649 - 4656.
[Abstract] [Full Text] [PDF]

R. G. Sargent, J. L. Meservy, B. D. Perkins, A. E. Kilburn, Z. Intody, G. M. Adair, R. S. Nairn, and J. H. Wilson
Role of the nucleotide excision repair gene ERCC1 in formation of recombination-dependent rearrangements in mammalian cells
Nucleic Acids Res., October 1, 2000; 28(19): 3771 - 3778.
[Abstract] [Full Text] [PDF]

D. Saxe, A. Datta, and S. Jinks-Robertson
Stimulation of Mitotic Recombination Events by High Levels of RNA Polymerase II Transcription in Yeast
Mol. Cell. Biol., August 1, 2000; 20(15): 5404 - 5414.
[Abstract] [Full Text]

B.-S. Lee, L. Bi, D. J. Garfinkel, and A. M. Bailis
Nucleotide Excision Repair/TFIIH Helicases Rad3 and Ssl2 Inhibit Short-Sequence Recombination and Ty1 Retrotransposition by Similar Mechanisms
Mol. Cell. Biol., April 1, 2000; 20(7): 2436 - 2445.
[Abstract] [Full Text]

T. Sedman, S. Kuusk, S. Kivi, and J. Sedman
A DNA Helicase Required for Maintenance of the Functional Mitochondrial Genome in Saccharomyces cerevisiae
Mol. Cell. Biol., March 1, 2000; 20(5): 1816 - 1824.
[Abstract] [Full Text]

S. Bärtsch, L. E. Kang, and L. S. Symington
RAD51 Is Required for the Repair of Plasmid Double-Stranded DNA Gaps from Either Plasmid or Chromosomal Templates
Mol. Cell. Biol., February 15, 2000; 20(4): 1194 - 1205.
[Abstract] [Full Text]

A. J. Rattray, B. K. Shafer, and D. J. Garfinkel
The Saccharomyces cerevisiae DNA Recombination and Repair Functions of the RAD52 Epistasis Group Inhibit Ty1 Transposition
Genetics, February 1, 2000; 154(2): 543 - 556.
[Abstract] [Full Text]

N. Erdeniz and R. Rothstein
Rsp5, a Ubiquitin-Protein Ligase, Is Involved in Degradation of the Single-Stranded-DNA Binding Protein Rfa1 in Saccharomyces cerevisiae
Mol. Cell. Biol., January 1, 2000; 20(1): 224 - 232.
[Abstract] [Full Text]

J. M. Murray and D. I. Johnson
Isolation and Characterization of Nrf1p, a Novel Negative Regulator of the Cdc42p GTPase in Schizosaccharomyces pombe
Genetics, January 1, 2000; 154(1): 155 - 165.
[Abstract] [Full Text]

Y. Bai, A. P. Davis, and L. S. Symington
A Novel Allele of RAD52 That Causes Severe DNA Repair and Recombination Deficiencies Only in the Absence of RAD51 or RAD59
Genetics, November 1, 1999; 153(3): 1117 - 1130.
[Abstract] [Full Text]

M. Fasullo, J. Koudelik, P. AhChing, P. Giallanza, and C. Cera
Radiosensitive and Mitotic Recombination Phenotypes of the Saccharomyces cerevisiae dun1 Mutant Defective in DNA Damage-Inducible Gene Expression
Genetics, July 1, 1999; 152(3): 909 - 919.
[Abstract] [Full Text]

J. Qiu, Y. Qian, V. Chen, M.-X. Guan, and B. Shen
Human Exonuclease 1 Functionally Complements Its Yeast Homologues in DNA Recombination, RNA Primer Removal, and Mutation Avoidance
J. Biol. Chem., June 18, 1999; 274(25): 17893 - 17900.
[Abstract] [Full Text] [PDF]

F. Paques and J. E. Haber
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 349 - 404.
[Abstract] [Full Text] [PDF]

T. Tsukiyama, J. Palmer, C. C. Landel, J. Shiloach, and C. Wu
Characterization of the Imitation Switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae
Genes & Dev., March 15, 1999; 13(6): 686 - 697.
[Abstract] [Full Text]

J. Smith and R. Rothstein
An Allele of RFA1 Suppresses RAD52-Dependent Double-Strand Break Repair in Saccharomyces cerevisiae
Genetics, February 1, 1999; 151(2): 447 - 458.
[Abstract] [Full Text]

S. Maines, M. C. Negritto, X. Wu, G. M. Manthey, and A. M. Bailis
Novel Mutations in the RAD3 and SSL1 Genes Perturb Genome Stability by Stimulating Recombination Between Short Repeats in Saccharomyces cerevisiae
Genetics, November 1, 1998; 150(3): 963 - 976.
[Abstract] [Full Text]

T. Sugiyama, J. H. New, and S. C. Kowalczykowski
DNA annealing by Rad52 Protein is stimulated by specific interaction with the complex of replication protein A and single-stranded DNA
PNAS, May 26, 1998; 95(11): 6049 - 6054.
[Abstract] [Full Text] [PDF]

N. Erdeniz, U. H. Mortensen, and R. Rothstein
Cloning-Free PCR-Based Allele Replacement Methods
Genome Res., December 1, 1997; 7(12): 1174 - 1183.
[Abstract] [Full Text] [PDF]

R. G. Sargent, R. L. Rolig, A. E. Kilburn, G. M. Adair, J. H. Wilson, and R. S. Nairn
Recombination-dependent deletion formation in mammalian cells deficient in the nucleotide excision repair gene ERCC1
PNAS, November 25, 1997; 94(24): 13122 - 13127.
[Abstract] [Full Text] [PDF]

J. E. Haber and W.-Y. Leung
Lack of chromosome territoriality in yeast: Promiscuous rejoining of broken chromosome ends
PNAS, November 26, 1996; 93(24): 13949 - 13954.
[Abstract] [Full Text] [PDF]

K. Rodriguez, Z. Wang, E. C. Friedberg, and A. E. Tomkinson
Identification of Functional Domains within the RAD1·RAD10 Repair and Recombination Endonuclease of Saccharomyces cerevisiae
J. Biol. Chem., August 23, 1996; 271(34): 20551 - 20558.
[Abstract] [Full Text] [PDF]

Y Bai and L S Symington
A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae.
Genes & Dev., August 15

				E. Mito, J. V. Mokhnatkin, M. C. Steele, V. L. Buettner, S. S. Sommer, G. M. Manthey, and A. M. Bailis Mutagenic and Recombinagenic Responses to Defective DNA Polymerase {delta} Are Facilitated by the Rev1 Protein in pol3-t Mutants of Saccharomyces cerevisiae Genetics, August 1, 2008; 179(4): 1795 - 1806. [Abstract] [Full Text] [PDF]

				S. Keppler-Ross, C. Noffz, and N. Dean A New Purple Fluorescent Color Marker for Genetic Studies in Saccharomyces cerevisiae and Candida albicans Genetics, May 1, 2008; 179(1): 705 - 710. [Abstract] [Full Text] [PDF]

				N. Averbeck, X.-D. Gao, S.-I. Nishimura, and N. Dean Alg13p, the Catalytic Subunit of the Endoplasmic Reticulum UDP-GlcNAc Glycosyltransferase, Is a Target for Proteasomal Degradation Mol. Biol. Cell, May 1, 2008; 19(5): 2169 - 2178. [Abstract] [Full Text] [PDF]

				L. Maloisel, F. Fabre, and S. Gangloff DNA Polymerase {delta} Is Preferentially Recruited during Homologous Recombination To Promote Heteroduplex DNA Extension Mol. Cell. Biol., February 15, 2008; 28(4): 1373 - 1382. [Abstract] [Full Text] [PDF]

				A. Montanari, C. Besagni, C. De Luca, V. Morea, R. Oliva, A. Tramontano, M. Bolotin-Fukuhara, L. Frontali, and S. Francisci Yeast as a model of human mitochondrial tRNA base substitutions: Investigation of the molecular basis of respiratory defects RNA, February 1, 2008; 14(2): 275 - 283. [Abstract] [Full Text] [PDF]

				S. Flott, C. Alabert, G. W. Toh, R. Toth, N. Sugawara, D. G. Campbell, J. E. Haber, P. Pasero, and J. Rouse Phosphorylation of Slx4 by Mec1 and Tel1 Regulates the Single-Strand Annealing Mode of DNA Repair in Budding Yeast Mol. Cell. Biol., September 15, 2007; 27(18): 6433 - 6445. [Abstract] [Full Text] [PDF]

				M. S. Navarro, L. Bi, and A. M. Bailis A Mutant Allele of the Transcription Factor IIH Helicase Gene, RAD3, Promotes Loss of Heterozygosity in Response to a DNA Replication Defect in Saccharomyces cerevisiae Genetics, July 1, 2007; 176(3): 1391 - 1402. [Abstract] [Full Text] [PDF]

				M. A. Barbera and T. D. Petes Selection and analysis of spontaneous reciprocal mitotic cross-overs in Saccharomyces cerevisiae PNAS, August 22, 2006; 103(34): 12819 - 12824. [Abstract] [Full Text] [PDF]

				K. C. Lindstrom, J. C. Vary Jr., M. R. Parthun, J. Delrow, and T. Tsukiyama Isw1 Functions in Parallel with the NuA4 and Swr1 Complexes in Stress-Induced Gene Repression. Mol. Cell. Biol., August 1, 2006; 26(16): 6117 - 6129. [Abstract] [Full Text] [PDF]

				A. Palomino, P. Herrero, and F. Moreno Tpk3 and Snf1 protein kinases regulate Rgt1 association with Saccharomyces cerevisiae HXK2 promoter Nucleic Acids Res., March 9, 2006; 34(5): 1427 - 1438. [Abstract] [Full Text] [PDF]

				B. O. Krogh, B. Llorente, A. Lam, and L. S. Symington Mutations in Mre11 Phosphoesterase Motif I That Impair Saccharomyces cerevisiae Mre11-Rad50-Xrs2 Complex Stability in Addition to Nuclease Activity Genetics, December 1, 2005; 171(4): 1561 - 1570. [Abstract] [Full Text] [PDF]

				X.-D. Gao, H. Tachikawa, T. Sato, Y. Jigami, and N. Dean Alg14 Recruits Alg13 to the Cytoplasmic Face of the Endoplasmic Reticulum to Form a Novel Bipartite UDP-N-acetylglucosamine Transferase Required for the Second Step of N-Linked Glycosylation J. Biol. Chem., October 28, 2005; 280(43): 36254 - 36262. [Abstract] [Full Text] [PDF]

				S. Huang, H. Zhou, D. Katzmann, M. Hochstrasser, E. Atanasova, and Z. Zhang Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing PNAS, September 20, 2005; 102(38): 13410 - 13415. [Abstract] [Full Text] [PDF]

				L. D. Langston and L. S. Symington Gene targeting in yeast is initiated by two independent strand invasions PNAS, October 26, 2004; 101(43): 15392 - 15397. [Abstract] [Full Text] [PDF]

				S. Nicholls, M. Straffon, B. Enjalbert, A. Nantel, S. Macaskill, M. Whiteway, and A. J. P. Brown Msn2- and Msn4-Like Transcription Factors Play No Obvious Roles in the Stress Responses of the Fungal Pathogen Candida albicans Eukaryot. Cell, October 1, 2004; 3(5): 1111 - 1123. [Abstract] [Full Text] [PDF]

				L. Krejci, M. Macris, Y. Li, S. Van Komen, J. Villemain, T. Ellenberger, H. Klein, and P. Sung Role of ATP Hydrolysis in the Antirecombinase Function of Saccharomyces cerevisiae Srs2 Protein J. Biol. Chem., May 28, 2004; 279(22): 23193 - 23199. [Abstract] [Full Text] [PDF]

				S. Grossi, A. Puglisi, P. V. Dmitriev, M. Lopes, and D. Shore Pol12, the B subunit of DNA polymerase {alpha}, functions in both telomere capping and length regulation Genes & Dev., May 1, 2004; 18(9): 992 - 1006. [Abstract] [Full Text] [PDF]

				T. A. Motycka, T. Bessho, S. M. Post, P. Sung, and A. E. Tomkinson Physical and Functional Interaction between the XPF/ERCC1 Endonuclease and hRad52 J. Biol. Chem., April 2, 2004; 279(14): 13634 - 13639. [Abstract] [Full Text] [PDF]

				T. Uchiki, L. T. Dice, R. L. Hettich, and C. Dealwis Identification of Phosphorylation Sites on the Yeast Ribonucleotide Reductase Inhibitor Sml1 J. Biol. Chem., March 19, 2004; 279(12): 11293 - 11303. [Abstract] [Full Text] [PDF]

				A. P. Davis and L. S. Symington RAD51-Dependent Break-Induced Replication in Yeast Mol. Cell. Biol., March 15, 2004; 24(6): 2344 - 2351. [Abstract] [Full Text] [PDF]

				J.-L. Ma, E. M. Kim, J. E. Haber, and S. E. Lee Yeast Mre11 and Rad1 Proteins Define a Ku-Independent Mechanism To Repair Double-Strand Breaks Lacking Overlapping End Sequences Mol. Cell. Biol., December 1, 2003; 23(23): 8820 - 8828. [Abstract] [Full Text] [PDF]

				P. A. Mieczkowski, J. O. Mieczkowska, M. Dominska, and T. D. Petes Genetic regulation of telomere-telomere fusions in the yeast Saccharomyces cerevisae PNAS, September 16, 2003; 100(19): 10854 - 10859. [Abstract] [Full Text] [PDF]

				A. Galli, T. Cervelli, and R. H. Schiestl Characterization of the Hyperrecombination Phenotype of the pol3-t Mutation of Saccharomyces cerevisiae Genetics, May 1, 2003; 164(1): 65 - 79. [Abstract] [Full Text] [PDF]

				M. M. Kessler, Q. Zeng, S. Hogan, R. Cook, A. J. Morales, and G. Cottarel Systematic Discovery of New Genes in the Saccharomyces cerevisiae Genome Genome Res., February 1, 2003; 13(2): 264 - 271. [Abstract] [Full Text] [PDF]

				J. C. Vary Jr., V. K. Gangaraju, J. Qin, C. C. Landel, C. Kooperberg, B. Bartholomew, and T. Tsukiyama Yeast Isw1p Forms Two Separable Complexes In Vivo Mol. Cell. Biol., January 1, 2003; 23(1): 80 - 91. [Abstract] [Full Text]

				L. S. Symington Role of RAD52 Epistasis Group Genes in Homologous Recombination and Double-Strand Break Repair Microbiol. Mol. Biol. Rev., December 1, 2002; 66(4): 630 - 670. [Abstract] [Full Text] [PDF]

				J. A. Freedman and S. Jinks-Robertson Genetic Requirements for Spontaneous and Transcription-Stimulated Mitotic Recombination in Saccharomyces cerevisiae Genetics, September 1, 2002; 162(1): 15 - 27. [Abstract] [Full Text] [PDF]

				G. M. Manthey and A. M. Bailis Multiple Pathways Promote Short-Sequence Recombination in Saccharomyces cerevisiae Mol. Cell. Biol., August 1, 2002; 22(15): 5347 - 5356. [Abstract] [Full Text] [PDF]

				M. C. V. L. Wong, S. R. S. Scott-Drew, M. J. Hayes, P. J. Howard, and J. A. H. Murray RSC2, Encoding a Component of the RSC Nucleosome Remodeling Complex, Is Essential for 2{micro}m Plasmid Maintenance in Saccharomyces cerevisiae Mol. Cell. Biol., June 15, 2002; 22(12): 4218 - 4229. [Abstract] [Full Text] [PDF]

				U. H. Mortensen, N. Erdeniz, Q. Feng, and R. Rothstein A Molecular Genetic Dissection of the Evolutionarily Conserved N Terminus of Yeast Rad52 Genetics, June 1, 2002; 161(2): 549 - 562. [Abstract] [Full Text] [PDF]