Genetic Resistance to Viral Infection: The Molecular Cloning of a Drosophila Gene That Restricts Infection by the Rhabdovirus Sigma

INVESTIGATIONS

Genetic Resistance to Viral Infection: The Molecular Cloning of a Drosophila Gene That Restricts Infection by the Rhabdovirus Sigma

D. Contamine, A. M. Petitjean and M. Ashburner
Laboratoire de Genetique des Virus, C.N.R.S., Gif-sur-Yvette, 91190 France

The ref(2)P gene of Drosophila melanogaster has two common alleles, ref(2)P(o) which permits the infection of flies by the rhabdovirus sigma ({sigma}), and ref(2)P(p) which is restrictive for {sigma} infection. This gene has been cloned by P element tagging and shown to code for two RNAs in adult flies. These RNAs are expressed in both males and females, but only the larger is expressed in ovaries. Both transcripts are shorter, by about 50 nucleotides, in flies carrying the ref(2)P(p) allele than in those carrying ref(2)P(o). The dominance relationships of these two alleles, and the fact that ref(2)P(null) alleles are permissive to {sigma} infection, suggest that the ref(2)P(o) product is antimorphic to that of the ref(2)P(p) allele.

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