Pachytene Arrest and Other Meiotic Effects of the Start Mutations in Saccharomyes cerevisiae

INVESTIGATIONS

Pachytene Arrest and Other Meiotic Effects of the Start Mutations in Saccharomyes cerevisiae

E. O. Shuster and B. Byers
Present address: Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143.

Mutations in the Start class of cell division cycle genes (CDC28, CDC36 and CDC39) define the point in the G(1) phase of the vegetative cycle at which the cell becomes committed to completing another round of cell division. Genetic, cytological and biochemical data demonstrate that these mutations cause meiotic cells to become arrested at pachytene following completion of both chromosomal DNA replication and spindle pole body (SPB) duplication. In contrast these mutations have previously been found to cause arrest of the mitotic cell cycle prior to either of these landmark events, so the role of the Start genes in these events during vegetative growth must be indirect. Our observations are consistent with the hypothesis that CDC28, CDC36 and CDC39 are required for irreversible commitment to nuclear division in both the mitotic and meiotic pathways. CDC28 was additionally found to be required for the SPB separation that precedes spindle formation in preparation for the second meiotic division. Cytological and genetic analyses of this requirement revealed both that such separation may fail independently at either SPB and that ascospore formation can proceed independently of SPB separation.

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				A. Sourirajan and M. Lichten Polo-like kinase Cdc5 drives exit from pachytene during budding yeast meiosis Genes & Dev., October 1, 2008; 22(19): 2627 - 2632. [Abstract] [Full Text] [PDF]

				L. Wan, H. Niu, B. Futcher, C. Zhang, K. M. Shokat, S. J. Boulton, and N. M. Hollingsworth Cdc28-Clb5 (CDK-S) and Cdc7-Dbf4 (DDK) collaborate to initiate meiotic recombination in yeast Genes & Dev., February 1, 2008; 22(3): 386 - 397. [Abstract] [Full Text] [PDF]

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				B. Mai and L. Breeden CLN1 and Its Repression by Xbp1 Are Important for Efficient Sporulation in Budding Yeast Mol. Cell. Biol., January 15, 2000; 20(2): 478 - 487. [Abstract] [Full Text]

				S. Parisi, M. J. McKay, M. Molnar, M. A. Thompson, P. J. van der Spek, E. van Drunen-Schoenmaker, R. Kanaar, E. Lehmann, J. H. J. Hoeijmakers, and J. Kohli Rec8p, a Meiotic Recombination and Sister Chromatid Cohesion Phosphoprotein of the Rad21p Family Conserved from Fission Yeast to Humans Mol. Cell. Biol., May 1, 1999; 19(5): 3515 - 3528. [Abstract] [Full Text] [PDF]

				M. D. Mendenhall and A. E. Hodge Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1191 - 1243. [Abstract] [Full Text] [PDF]

				S. R. Hepworth, H. Friesen, and J. Segall NDT80 and the Meiotic Recombination Checkpoint Regulate Expression of Middle Sporulation-Specific Genes in Saccharomyces cerevisiae Mol. Cell. Biol., October 1, 1998; 18(10): 5750 - 5761. [Abstract] [Full Text]

				L. Dirick, L. Goetsch, G. Ammerer, and B. Byers Regulation of Meiotic S Phase by Ime2 and a Clb5,6-Associated Kinase in Saccharomyces cerevisiae Science, September 18, 1998; 281(5384): 1854 - 1857. [Abstract] [Full Text]

				D. Stuart and C. Wittenberg CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint Genes & Dev., September 1, 1998; 12(17): 2698 - 2710. [Abstract] [Full Text]

				L Xu, B M Weiner, and N Kleckner Meiotic cells monitor the status of the interhomolog recombination complex. Genes & Dev., January 1, 1997; 11(1): 106 - 118. [Abstract] [PDF]

				C. Eberhart and S. Wasserman The pelota locus encodes a protein required for meiotic cell division: an analysis of G2/M arrest in Drosophila spermatogenesis Development, January 10, 1995; 121(10): 3477 - 3486. [Abstract] [PDF]

				L Krisak, R Strich, R S Winters, J P Hall, M J Mallory, D Kreitzer, R S Tuan, and E Winter SMK1, a developmentally regulated MAP kinase, is required for spore wall assembly in Saccharomyces cerevisiae. Genes & Dev., September 15, 1994; 8(18): 2151 - 2161. [Abstract] [PDF]

				H Friesen, R Lunz, S Doyle, and J Segall Mutation of the SPS1-encoded protein kinase of Saccharomyces cerevisiae leads to defects in transcription and morphology during spore formation. Genes & Dev., September 15, 1994; 8(18): 2162 - 2175. [Abstract] [PDF]

				M A Collart and K Struhl NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization. Genes & Dev., March 1, 1994; 8(5): 525 - 537. [Abstract] [PDF]

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