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Genetics, Vol 122, 331-339, Copyright © 1989
INVESTIGATIONS |
Characterization of the amdR-Controlled lamA and lamB Genes of Aspergillus nidulans
M. E. Katz and M. J. Hynes
Present address: Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia.
Four Aspergillus nidulans genes are known to be under the control of the trans-acting regulatory gene amdR. We describe the isolation and initial characterization of one of these amdR-regulated genes, lamA. The lam locus, however, was found to consist of two divergently transcribed genes, the lamA gene, and a new gene, also under amdR control, which we have designated lamB. Using recombinant DNA techniques we have constructed a strain of A. nidulans lacking a functional lamB gene. Experiments conducted with this strain demonstrate that lamB, like lamA, is involved in utilization of 2-pyrrolidinone in A. nidulans. Metabolism of a related compound, {gamma}-amino butyric acid (GABA) is not affected. We also provide evidence that the conversion of exogenous 2-pyrrolidinone to endogenous GABA requires a functional lamB gene. The expression of both lamA and lamB is subject to carbon and nitrogen metabolite repression in addition to amdR-mediated induction by {omega}-amino acids.
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