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Genetics, Vol 122, 279-296, Copyright © 1989
INVESTIGATIONS |
Genetic and Physical Mapping of the mcrA (rglA) and mcrB (rglB) Loci of Escherichia coli K-12
E. A. Raleigh, R. Trimarchi and H. Revel
New England BioLabs, Beverly, Massachusetts 01915-9990
We have genetically analyzed, cloned and physically mapped the modified cytosine-specific restriction determinants mcrA (rglA) and mcrB (rglB) of Escherichia coli K-12. The independently discovered Rgl and Mcr restriction systems are shown to be identical by three criteria: 1) mutants with the RglA(-) or RglB(-) phenotypes display the corresponding McrA(-) or McrB(-) phenotypes, and vice versa; 2) the gene(s) for RglA and McrA reside together at one locus, while gene(s) for RglB and McrB are coincident at a different locus; and 3) RglA(+) and RglB(+) recombinant clones complement for the corresponding Mcr-deficient lesions. The mcrA (rglA) gene(s) is on the excisable element e14, just clockwise of purB at 25 min. The mcrB (rglB) gene(s), at 99 min, is in a cluster of restriction functions that includes hsd and mrr, determinants of host-specific restriction (EcoK) and methyladenine-specific restriction respectively. Gene order is mcrB-hsdS-hsdM-hsdR-mrr-serB. Possible models for the acqusition of these restriction determinants by enteric bacteria are discussed.
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