- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Luisi-DeLuca, C.
- Articles by Kolodner, R. D.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Luisi-DeLuca, C.
- Articles by Kolodner, R. D.
Genetics, Vol 122, 269-278, Copyright © 1989
INVESTIGATIONS |
Genetic and Physical Analysis of Plasmid Recombination in recB recC sbcB and recB recC sbcA Escherichia coli K-12 Mutants
C. Luisi-DeLuca, S. T. Lovett and R. D. Kolodner
Laboratory of Molecular Genetics, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
The effect of mutations in known recombination genes (recA, recB, recC, recE, recF, recJ, recN, recO, recQ and ruv) on intramolecular recombination of plasmids was studied in recB recC sbcB and recB recC sbcA Escherichia coli mutants. The rate of recombination of circular dimer plasmids was at least 1000-fold higher in recB recC sbcB or recB recC sbcA mutants as compared to wild-type cells. The rate was decreased by mutations in recA, recF, recJ, recO, ruv or mutS in recB recC sbcB mutants, and by mutations in recE, recN, recO, recQ, ruv or mutS in recB recC sbcA mutants. In addition to measuring the recombination rate of circular dimer plasmids, the recombination-mediated transformation of linear dimer plasmids was also studied. Linear dimer plasmids transformed recB recC sbcB and recB recC sbcA mutants 20- to 40-fold more efficiently than wild-type cells. The transformation efficiency of linear dimer plasmids in recB recC sbcB mutants was decreased by mutations in recA, recF, recJ, recO, recQ or lexA (lexA3). In recB recC sbcA mutants the transformation efficiency of linear dimers was decreased only by a recE mutation. Physical analysis of linear dimer- or circular dimer-transformed recB recC sbcB mutants revealed that all transformants contained recombinant monomer genotypes. This suggests that recombination in recB recC sbcB cells is very efficient.
This article has been cited by other articles:
 |
|
 |
|
  A.-M. Khalil, J. A. Julius, and J. Bachant One step construction of PCR mutagenized libraries for genetic analysis by recombination cloning Nucleic Acids Res., August 15, 2007; (2007) gkm583v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. Wang, B. K.C. Lo, and G. Winter Generating molecular diversity by homologous recombination in Escherichia coli Protein Eng. Des. Sel., August 1, 2005; 18(8): 397 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zuniga-Castillo, D. Romero, and J. M. Martinez-Salazar The Recombination Genes addAB Are Not Restricted to Gram-Positive Bacteria: Genetic Analysis of the Recombination Initiation Enzymes RecF and AddAB in Rhizobium etli J. Bacteriol., December 1, 2004; 186(23): 7905 - 7913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Bernstein and J. L. Keck Domain mapping of Escherichia coli RecQ defines the roles of conserved N- and C-terminal regions in the RecQ family Nucleic Acids Res., June 1, 2003; 31(11): 2778 - 2785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Beam, C. J. Saveson, and S. T. Lovett Role for radA/sms in Recombination Intermediate Processing in Escherichia coli J. Bacteriol., December 15, 2002; 184(24): 6836 - 6844. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bzymek and S. T. Lovett Instability of repetitive DNA sequences: The role of replication in multiple mechanisms PNAS, July 17, 2001; 98(15): 8319 - 8325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bzymek and S. T. Lovett Evidence for Two Mechanisms of Palindrome-Stimulated Deletion in Escherichia coli: Single-Strand Annealing and Replication Slipped Mispairing Genetics, June 1, 2001; 158(2): 527 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kuzminov Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage lambda Microbiol. Mol. Biol. Rev., December 1, 1999; 63(4): 751 - 813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Saveson and S. T. Lovett Tandem Repeat Recombination Induced by Replication Fork Defects in Escherichia coli Requires a Novel Factor, RadC Genetics, May 1, 1999; 152(1): 5 - 13. [Abstract] [Full Text]
|
|
|
|
|
|
P. Zavodszky, J. Kardos, A. Svingor, and G. A. Petsko Adjustment of conformational flexibility is a key event in the thermal adaptation of proteins PNAS, June 23, 1998; 95(13): 7406 - 7411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Liu, A.-J. Cheng, and T.-c. V. Wang Involvement of recF, recO, and recR Genes in UV-Radiation Mutagenesis of Escherichia coli J. Bacteriol., April 1, 1998; 180(7): 1766 - 1770. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. E. Iype, R. B. Inman, and M. M. Cox Blocked RecA Protein-mediated DNA Strand Exchange Reactions Are Reversed by the RuvA and RuvB Proteins J. Biol. Chem., August 18, 1995; 270(33): 19473 - 19480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Svingor, J. Kardos, I. Hajdu, A. Nemeth, and P. Zavodszky A Better Enzyme to Cope with Cold. COMPARATIVE FLEXIBILITY STUDIES ON PSYCHROTROPHIC, MESOPHILIC, AND THERMOPHILIC IPMDHS J. Biol. Chem., July 20, 2001; 276(30): 28121 - 28125. [Abstract] [Full Text] [PDF]
|
|