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Genetics, Vol 119, 751-757, Copyright © 1988
INVESTIGATIONS |
Double-Stranded Gap Repair of DNA by Gene Conversion in Escherichia coli
I. Kobayashi and N. Takahashi
Department of Bacteriology, Faculty of Medicine, University of Tokyo, Hongo, Tokyo 113, Japan, and National Children's Medical Research Center, Taishido, Tokyo 154, Japan
We demonstrated repair of a double-stranded DNA gap through gene conversion by a homologous DNA sequence in Escherichia coli. We made a double-stranded gap in one of the two regions of homology in an inverted orientation on a plasmid DNA molecule and introduced it into an E. coli strain which has the RecE system of recombination (genotype; sbcA23 recB21 recC22). We detected repair products by genetic selection. The repair products were those expected by the double-strand-gap repair model. Gene conversion was frequently accompanied by crossing over of the flanking sequences as in eukaryotes. This double-strand gap repair mechanism can explain plasmid recombination in the absence of an artificial double-stranded break reported in a companion study by Yamamoto et al.
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