Genetic and Physical Analysis of the M26 Recombination Hotspot of Schizosaccharomyces pombe

INVESTIGATIONS

Genetic and Physical Analysis of the M26 Recombination Hotspot of Schizosaccharomyces pombe

A. S. Ponticelli, E. P. Sena and G. R. Smith
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, and Department of Pathology, University of Washington, Seattle, Washington 98155

The ade6-M26 mutation of Schizosaccharomyces pombe has previously been reported to stimulate ade6 intragenic meiotic recombination. We report here that the ade6-M26 mutation is a single G -> T nucleotide change, that M26 stimulated recombination within ade6 but not at other distant loci, and that M26 stimulated meiotic but not mitotic recombination. In addition, M26 stimulated recombination within ade6 when M26 is homozygous; this result demonstrates that a base-pair mismatch at the M26 site was not required for the stimulation. These results are consistent with the ade6-M26 mutation creating a meiotic recombination initiation site.

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				P. J. Yeadon, F. J. Bowring, and D. E. A. Catcheside Alleles of the Hotspot cog Are Codominant in Effect on Recombination in the his-3 Region of Neurospora Genetics, July 1, 2004; 167(3): 1143 - 1153. [Abstract] [Full Text] [PDF]

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				K. Hirota, C. S. Hoffman, T. Shibata, and K. Ohta Fission Yeast Tup1-Like Repressors Repress Chromatin Remodeling at the fbp1+ Promoter and the ade6-M26 Recombination Hotspot Genetics, October 1, 2003; 165(2): 505 - 515. [Abstract] [Full Text] [PDF]

				J. A. Farah, E. Hartsuiker, K.-i. Mizuno, K. Ohta, and G. R. Smith A 160-bp Palindrome Is a Rad50{middle dot}Rad32-Dependent Mitotic Recombination Hotspot in Schizosaccharomyces pombe Genetics, May 1, 2002; 161(1): 461 - 468. [Abstract] [Full Text] [PDF]

				L. Davis and G. R. Smith Meiotic recombination and chromosome segregation in Schizosaccharomyces pombe PNAS, July 17, 2001; 98(15): 8395 - 8402. [Abstract] [Full Text] [PDF]

				J. B. Virgin, J. P. Bailey, F. Hasteh, J. Neville, A. Cole, and G. Tromp Crossing Over Is Rarely Associated With Mitotic Intragenic Recombination in Schizosaccharomyces pombe Genetics, January 1, 2001; 157(1): 63 - 77. [Abstract] [Full Text]

				M. E. Fox, T. Yamada, K. Ohta, and G. R. Smith A Family of cAMP-Response-Element-Related DNA Sequences With Meiotic Recombination Hotspot Activity in Schizosaccharomyces pombe Genetics, September 1, 2000; 156(1): 59 - 68. [Abstract] [Full Text]

				A. Yamamoto, R. R. West, J. R. McIntosh, and Y. Hiraoka A Cytoplasmic Dynein Heavy Chain Is Required for Oscillatory Nuclear Movement of Meiotic Prophase and Efficient Meiotic Recombination in Fission Yeast J. Cell Biol., June 14, 1999; 145(6): 1233 - 1250. [Abstract] [Full Text] [PDF]

				G. Thon, K. P. Bjerling, and I. S. Nielsen Localization and Properties of a Silencing Element Near the mat3-M Mating-Type Cassette of Schizosaccharomyces pombe Genetics, March 1, 1999; 151(3): 945 - 963. [Abstract] [Full Text]

				C. Rudolph, C. Kunz, S. Parisi, E. Lehmann, E. Hartsuiker, B. Fartmann, W. Kramer, J. Kohli, and O. Fleck The msh2 Gene of Schizosaccharomyces pombe Is Involved in Mismatch Repair, Mating-Type Switching, and Meiotic Chromosome Organization Mol. Cell. Biol., January 1, 1999; 19(1): 241 - 250. [Abstract] [Full Text] [PDF]

				N. Kon, S. C. Schroeder, M. D. Krawchuk, and W. P. Wahls Regulation of the Mts1-Mts2-Dependent ade6-M26 Meiotic Recombination Hot Spot and Developmental Decisions by the Spc1 Mitogen-Activated Protein Kinase of Fission Yeast Mol. Cell. Biol., December 1, 1998; 18(12): 7575 - 7583. [Abstract] [Full Text]

				J. B. Virgin and J. P. Bailey The M26 Hotspot of Schizosaccharomyces pombe Stimulates Meiotic Ectopic Recombination and Chromosomal Rearrangements Genetics, July 1, 1998; 149(3): 1191 - 1204. [Abstract] [Full Text] [PDF]

				A. Nicolas Relationship between transcription and initiation of meiotic recombination: Toward chromatin accessibility PNAS, January 6, 1998; 95(1): 87 - 89. [Full Text] [PDF]

				N. Kon, M. D. Krawchuk, B. G. Warren, G. R. Smith, and W. P. Wahls Transcription factor Mts1/Mts2 (Atf1/Pcr1, Gad7/Pcr1) activates the M26 meiotic recombination hotspot in pombe PNAS, December 9, 1997; 94(25): 13765 - 13770. [Abstract] [Full Text] [PDF]

				M. E. Fox, J. B. Virgin, J. Metzger, and G. R. Smith Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 PNAS, July 8, 1997; 94(14): 7446 - 7451. [Abstract] [Full Text] [PDF]

				K Mizuno, Y Emura, M Baur, J Kohli, K Ohta, and T Shibata The meiotic recombination hot spot created by the single-base substitution ade6-M26 results in remodeling of chromatin structure in fission yeast. Genes & Dev., April 1, 1997; 11(7): 876 - 886. [Abstract] [PDF]

				W P Wahls and G R Smith A heteromeric protein that binds to a meiotic homologous recombination hot spot: correlation of binding and hot spot activity. Genes & Dev., July 15, 1994; 8(14): 1693 - 1702. [Abstract] [PDF]

				L C DeVeaux and G R Smith Region-specific activators of meiotic recombination in Schizosaccharomyces pombe. Genes & Dev., January 15, 1994; 8(2): 203 - 210. [Abstract] [PDF]