The unc-22(IV) Region of Caenorhabditis elegans: Genetic Analysis of Lethal Mutations

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The unc-22(IV) Region of Caenorhabditis elegans: Genetic Analysis of Lethal Mutations

D. V. Clark, T. M. Rogalski, L. M. Donati and D. L. Baillie
Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6

The organization of essential genes in the unc-22 region, defined by the deficiency sDf2 on linkage group IV, has been studied. Using the balancer nT1 (IV;V), which suppresses recombination over 49 map units, 294 lethal mutations on LGIV(right) and LGV(left) were recovered using EMS mutagenesis. Twenty-six of these mutations fell into the unc-22 region. Together with previously isolated lethal mutations, there is now a total of 63 lethal mutations which fall into 31 complementation groups. Mutations were positioned on the map using eight overlapping deficiencies in addition to sDf2. The lethal alleles and deficiencies in the unc-22 region were characterized with respect to their terminal phenotypes. Mapping of these lethal mutations shows that sDf2 deletes a minimum of 1.8 map units and a maximum of 2.5 map units. A minimum estimate of essential gene number for the region using a truncated Poisson calculation is 48. The data indicate a minimum estimate of approximately 3500 essential genes in the Caenorhabditis elegans genome.

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