- THIS ARTICLE
- Full Text (PDF)
- A corrigendum has been published
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Lund, S. D.
- Articles by Ganschow, R. E.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Lund, S. D.
- Articles by Ganschow, R. E.
Genetics, Vol 119, 151-156, Copyright © 1988
INVESTIGATIONS |
Androgen Regulation of Murine {beta}-Glucuronidase Expression: Identification and Characterization of a Nonresponse Variant
S. D. Lund, D. Miller, V. Chapman and R. E. Ganschow
Division of Basic Science Research, Children's Hospital Research Foundation, Cincinnati, Ohio 45229, Graduate Program in Developmental Biology, University of Cincinnati, Cincinnati, Ohio 45221
One of the major features of {beta}-glucuronidase (GUS) expression in inbred strains of the house mouse, Mus musculus, is the responsiveness of this enzyme to androgen stimulation in tubule cells of the kidney. Both GUS-specific and nonspecific mutations have been described which define genes that serve to control this response. During examination of the expression of GUS in the interbreeding subspecies, Mus hortulanus, a new GUS haplotype was uncovered that is characterized, in part, by a lack of GUS response to androgen stimulation in an apparently responsive kidney. Blot hybridization analyses of kidney RNA with a radiolabeled murine GUS cDNA shows this lack of response to be reflected in GUS mRNA levels. The difference in heat stability of GUS activity between M. hortulanus and a responsive inbred strain, ICR/Ha, was utilized to assess the contribution of each parent to kidney levels of GUS in androgen-treated and -untreated F(1) progeny of these strains. The results, together with preliminary genetic studies, suggest that the element controlling this responsiveness (or the lack thereof) is cis-active and tightly linked to the GUS structural gene on chromosome 5. It is not known whether this element is identical to another GUS-specific, cis-active element, Gus-r, which also controls the androgen response of GUS in mouse kidney.
This article has been cited by other articles:
 |
|
 |
|
  P. Val, A. Martinez, I. Sahut-Barnola, C. Jean, G. Veyssiere, and A.-M. Lefrancois-Martinez A 77-Base Pair LINE-Like Sequence Elicits Androgen-Dependent mvdp/akr1-b7 Expression in Mouse Vas Deferens, But Is Dispensable for Adrenal Expression in Rats Endocrinology, September 1, 2002; 143(9): 3435 - 3448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Thornton, D. W. Thomas, P. M. Gallagher, and R. E. Ganschow Androgen Responsiveness of Mouse Kidney {beta}-Glucuronidase Requires 5'-Flanking and Intragenic Gus-s Sequences Mol. Endocrinol., March 1, 1998; 12(3): 333 - 341. [Abstract] [Full Text]
|
|
|
|
|
|
M J. Melia, N. Bofill, M. Hubank, and A. Meseguer Identification of Androgen-Regulated Genes in Mouse Kidney by Representational Difference Analysis and Random Arbitrarily Primed Polymerase Chain Reaction Endocrinology, February 1, 1998; 139(2): 688 - 695. [Abstract] [Full Text] [PDF]
|
|