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Genetics, Vol 119, 13-20, Copyright © 1988
INVESTIGATIONS |
Multiple New Genes That Determine Activity for the First Step of Leucine Biosynthesis in Saccharomyces cerevisiae
P. Drain and P. Schimmel
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
The first step in the biosynthesis of leucine is catalyzed by {alpha}-isopropylmalate ({alpha}-IPM) synthase. In the yeast Saccharomyces cerevisiae, LEU4 encodes the isozyme responsible for the majority of {alpha}-IPM synthase activity. Yeast strains that bear disruption alleles of LEU4, however, are Leu(+) and exhibit a level of synthase activity that is 20% of the wild type. To identify the gene or genes that encode this remaining activity, a leu4 disruption strain was mutagenized. The mutations identified define three new complementation groups, designated leu6, leu7 and leu8. Each of these new mutations effect leucine auxotrophy only if a leu4 mutation is present and each results in loss of {alpha}-IPM synthase activity. Further analysis suggests that LEU7 and LEU8 are candidates for the gene or genes that encode an {alpha}-IPM synthase activity. The results demonstrate that multiple components determine the residual {alpha}-IPM synthase activity in leu4 gene disruption strains of S. cerevisiae.
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