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Genetics, Vol 118, 601-607, Copyright © 1988
INVESTIGATIONS |
Nonsense Mutations in Essential Genes of Saccharomyces cerevisiae
L. Riles and M. V. Olson
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110
A new method for isolating nonsense mutations in essential yeast genes has been used to develop a collection of 115 ochre mutations that define 94 complementation groups. The mutants are isolated in a genetic background that includes an ochre suppressor on a metastable plasmid and a suppressible colony-color marker on a chromosome. When the parental strain is plated on a rich medium, the colonies display a pattern of red, plasmid-free sectors on a white background. Mutants containing an ochre mutation in any essential yeast gene give rise to nonsectoring, white colonies, since cell growth is dependent on the presence of the plasmid-borne suppressor. Analysis of the data suggests that mutations are being recovered from a pool of approximately 250 genes.
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