Functional Interactions of Neurogenic Genes of Drosophila melanogaster

INVESTIGATIONS

Functional Interactions of Neurogenic Genes of Drosophila melanogaster

A. de-la-Concha, U. Dietrich, D. Weigel and J. A. Campos-Ortega
Present address: Departamento de Genetica, Facultad de Biologia, Universidad de Murcia, Murcia, Spain.

The neurogenic genes of Drosophila melanogaster are involved in the decision of ectodermal cells to take on a neural or an epidermal fate. We present evidence in support of the notion that six of the neurogenic genes are functionally related. We studied the phenotype of embryos lacking one of the neurogenic genes in the presence of an increased dosage of the wild-type allele of another neurogenic gene. Our analysis also included the Hairless locus, whose function is related to that of the neurogenic genes, as well as to many other genes. The effects observed were asymmetric in that triploidy for a given gene modified the phenotype of loss of the function of another gene, but triploidy of the latter gene did not modify the phenotype of loss of the function of the former gene. These asymmetries allowed us to establish a polarity of gene interactions, as well as to order the genes according to the assumed ability of some of them to modify the activity of others. In this sequence, almondex is the first link and Enhancer of split the last one. Our evidence suggests that the function of big brain is independent of the function of the other six. The consequences of this arrangement for the commitment of ectodermal cells are discussed.

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				B. Vollrath, J. Pudney, S. Asa, P. Leder, and K. Fitzgerald Isolation of a Murine Homologue of the Drosophila neuralized Gene, a Gene Required for Axonemal Integrity in Spermatozoa and Terminal Maturation of the Mammary Gland Mol. Cell. Biol., November 1, 2001; 21(21): 7481 - 7494. [Abstract] [Full Text] [PDF]

				J. de Celis and S. Bray The Abruptex domain of Notch regulates negative interactions between Notch, its ligands and Fringe Development, January 3, 2000; 127(6): 1291 - 1302. [Abstract] [PDF]

				C Takke, P Dornseifer, E v Weizsacker, and J. Campos-Ortega her4, a zebrafish homologue of the Drosophila neurogenic gene E(spl), is a target of NOTCH signalling Development, January 5, 1999; 126(9): 1811 - 1821. [Abstract] [PDF]

				M. J. Go and S. Artavanis-Tsakonas A Genetic Screen for Novel Components of the Notch Signaling Pathway During Drosophila Bristle Development Genetics, September 1, 1998; 150(1): 211 - 220. [Abstract] [Full Text]

				J. Culí and J. Modolell Proneural gene self-stimulation in neural precursors: an essential mechanism for sense organ development that is regulated by Notch signaling Genes & Dev., July 1, 1998; 12(13): 2036 - 2047. [Abstract] [Full Text]

				B. Kehl, K. Cho, and K. Choi mirror, a Drosophila homeobox gene in the Iroquois complex, is required for sensory organ and alula formation Development, January 4, 1998; 125(7): 1217 - 1227. [Abstract] [PDF]

				B. Giebel and J. A. Campos-Ortega Functional dissection of the Drosophila Enhancer of split protein, a suppressor of neurogenesis PNAS, June 10, 1997; 94(12): 6250 - 6254. [Abstract] [Full Text] [PDF]

				S Wang, S Younger-Shepherd, L. Jan, and Y. Jan Only a subset of the binary cell fate decisions mediated by Numb/Notch signaling in Drosophila sensory organ lineage requires Suppressor of Hairless Development, January 11, 1997; 124(22): 4435 - 4446. [Abstract] [PDF]

				D Doherty, L. Jan, and Y. Jan The Drosophila neurogenic gene big brain, which encodes a membrane-associated protein, acts cell autonomously and can act synergistically with Notch and Delta Development, January 10, 1997; 124(19): 3881 - 3893. [Abstract] [PDF]

				J. de Celis, S Bray, and A Garcia-Bellido Notch signalling regulates veinlet expression and establishes boundaries between veins and interveins in the Drosophila wing Development, January 5, 1997; 124(10): 1919 - 1928. [Abstract] [PDF]

				C. Micchelli, E. Rulifson, and S. Blair The function and regulation of cut expression on the wing margin of Drosophila: Notch, Wingless and a dominant negative role for Delta and Serrate Development, January 4, 1997; 124(8): 1485 - 1495. [Abstract] [PDF]

				J. de Celis, J de Celis, P Ligoxygakis, A Preiss, C Delidakis, and S Bray Functional relationships between Notch, Su(H) and the bHLH genes of the E(spl) complex: the E(spl) genes mediate only a subset of Notch activities during imaginal development Development, January 9, 1996; 122(9): 2719 - 2728. [Abstract] [PDF]

				R Baker and G Schubiger Autonomous and nonautonomous Notch functions for embryonic muscle and epidermis development in Drosophila Development, January 2, 1996; 122(2): 617 - 626. [Abstract] [PDF]

				J. de Celis, A Garcia-Bellido, and S. Bray Activation and function of Notch at the dorsal-ventral boundary of the wing imaginal disc Development, January 1, 1996; 122(1): 359 - 369. [Abstract] [PDF]

				M Lecourtois and F Schweisguth The neurogenic suppressor of hairless DNA-binding protein mediates the transcriptional activation of the enhancer of split complex genes triggered by Notch signaling. Genes & Dev., November 1, 1995; 9(21): 2598 - 2608. [Abstract] [PDF]

				A M Bailey and J W Posakony Suppressor of hairless directly activates transcription of enhancer of split complex genes in response to Notch receptor activity. Genes & Dev., November 1, 1995; 9(21): 2609 - 2622. [Abstract] [PDF]

				S Artavanis-Tsakonas, K Matsuno, and M. Fortini Notch signaling Science, April 14, 1995; 268(5208): 225 - 232. [Abstract] [PDF]

				C Oka, T Nakano, A Wakeham, J. de la Pompa, C Mori, T Sakai, S Okazaki, M Kawaichi, K Shiota, T. Mak, et al. Disruption of the mouse RBP-J kappa gene results in early embryonic death Development, January 10, 1995; 121(10): 3291 - 3301. [Abstract] [PDF]

				K Matsuno, R. Diederich, M. Go, C. Blaumueller, and S Artavanis-Tsakonas Deltex acts as a positive regulator of Notch signaling through interactions with the Notch ankyrin repeats Development, January 8, 1995; 121(8): 2633 - 2644. [Abstract] [PDF]

				F Schweisguth Suppressor of Hairless is required for signal reception during lateral inhibition in the Drosophila pupal notum Development, January 6, 1995; 121(6): 1875 - 1884. [Abstract] [PDF]

				Y Gu, N. Hukriede, and R. Fleming Serrate expression can functionally replace Delta activity during neuroblast segregation in the Drosophila embryo Development, January 3, 1995; 121(3): 855 - 865. [Abstract] [PDF]

				M. Martin-Bermudo, A Carmena, and F Jimenez Neurogenic genes control gene expression at the transcriptional level in early neurogenesis and in mesectoderm specification Development, January 1, 1995; 121(1): 219 - 224. [Abstract] [PDF]

				C Brou, F Logeat, M Lecourtois, J Vandekerckhove, P Kourilsky, F Schweisguth, and A Israel Inhibition of the DNA-binding activity of Drosophila suppressor of hairless and of its human homolog, KBF2/RBP-J kappa, by direct protein-protein interaction with Drosophila hairless. Genes & Dev., October 15, 1994; 8(20): 2491 - 2503. [Abstract] [PDF]

				B Jennings, A Preiss, C Delidakis, and S Bray The Notch signalling pathway is required for Enhancer of split bHLH protein expression during neurogenesis in the Drosophila embryo Development, January 12, 1994; 120(12): 3537 - 3548. [Abstract] [PDF]

				B Kramatschek and J. Campos-Ortega Neuroectodermal transcription of the Drosophila neurogenic genes E(spl) and HLH-m5 is regulated by proneural genes Development, January 4, 1994; 120(4): 815 - 826. [Abstract] [PDF]

				T. Menne and C Klambt The formation of commissures in the Drosophila CNS depends on the midline cells and on the Notch gene Development, January 1, 1994; 120(1): 123 - 133. [Abstract] [PDF]

				T Lieber, S Kidd, E Alcamo, V Corbin, and M W Young Antineurogenic phenotypes induced by truncated Notch proteins indicate a role in signal transduction and may point to a novel function for Notch in nuclei. Genes & Dev., October 1, 1993; 7(10): 1949 - 1965. [Abstract] [PDF]

				E Giniger, L. Jan, and Y. Jan Specifying the path of the intersegmental nerve of the Drosophila embryo: a role for Delta and Notch Development, January 2, 1993; 117(2): 431 - 440. [Abstract] [PDF]

				P. Kooh, R. Fehon, and M. Muskavitch Implications of dynamic patterns of Delta and Notch expression for cellular interactions during Drosophila development Development, January 2, 1993; 117(2): 493 - 507. [Abstract] [PDF]