Genetics, Vol 118, 417-423, Copyright © 1988

INVESTIGATIONS

Nitrate Reduction Mutants of Fusarium moniliforme (Gibberella fujikuroi)

CJR. Klittich and J. F. Leslie
Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506

Twelve strains of Fusarium moniliforme were examined for their ability to sector spontaneously on toxic chlorate medium. All strains sectored frequently; 91% of over 1200 colonies examined formed chlorate-resistant, mutant sectors. Most of these mutants had lesions in the nitrate reduction pathway and were unable to utilize nitrate (nit mutants). nit mutations occurred in seven loci: a structural gene for nitrate reductase (nit1), a regulatory gene specific for the nitrate reduction pathway (nit3), and five genes controlling the production of a molybdenum-containing cofactor that is necessary for nitrate reductase activity (nit2, nit4, nit5, nit6, nit7). No mutations affecting nitrite reductase or a major nitrogen regulatory locus were found among over 1000 nit mutants. Mutations of nit1 were recovered most frequently (39-66%, depending on the strain) followed by nit3 mutations (23-42%). The frequency of isolation of each mutant type could be altered, however, by changing the source of nitrogen in the chlorate medium. We concluded that genetic control of nitrate reduction in F. moniliforme is similar to that in Aspergillus and Neurospora, but that the overall regulation of nitrogen metabolism may be different.

This article has been cited by other articles:

				J. Lee, J. E. Jurgenson, J. F. Leslie, and R. L. Bowden Alignment of Genetic and Physical Maps of Gibberella zeae Appl. Envir. Microbiol., April 15, 2008; 74(8): 2349 - 2359. [Abstract] [Full Text] [PDF]

				H. E. Hallen and F. Trail The L-Type Calcium Ion Channel Cch1 Affects Ascospore Discharge and Mycelial Growth in the Filamentous Fungus Gibberella zeae (Anamorph Fusarium graminearum) Eukaryot. Cell, February 1, 2008; 7(2): 415 - 424. [Abstract] [Full Text] [PDF]

				Z. Kerenyi, B. Olah, A. Jeney, L. Hornok, and J. F. Leslie The Homologue of het-c of Neurospora crassa Lacks Vegetative Compatibility Function in Fusarium proliferatum. Appl. Envir. Microbiol., October 1, 2006; 72(10): 6527 - 6532. [Abstract] [Full Text] [PDF]

				J.-E. Kim, J. Jin, H. Kim, J.-C. Kim, S.-H. Yun, and Y.-W. Lee GIP2, a Putative Transcription Factor That Regulates the Aurofusarin Biosynthetic Gene Cluster in Gibberella zeae Appl. Envir. Microbiol., February 1, 2006; 72(2): 1645 - 1652. [Abstract] [Full Text] [PDF]

				P. C. E. Lepoint, F. T. J. Munaut, and H. M. M. Maraite Gibberella xylarioides Sensu Lato from Coffea canephora: a New Mating Population in the Gibberella fujikuroi Species Complex Appl. Envir. Microbiol., December 1, 2005; 71(12): 8466 - 8471. [Abstract] [Full Text] [PDF]

				I. Gaffoor, D. W. Brown, R. Plattner, R. H. Proctor, W. Qi, and F. Trail Functional Analysis of the Polyketide Synthase Genes in the Filamentous Fungus Gibberella zeae (Anamorph Fusarium graminearum) Eukaryot. Cell, November 1, 2005; 4(11): 1926 - 1933. [Abstract] [Full Text] [PDF]

				L. R. Gale, J. D. Bryant, S. Calvo, H. Giese, T. Katan, K. O'Donnell, H. Suga, M. Taga, T. R. Usgaard, T. J. Ward, et al. Chromosome Complement of the Fungal Plant Pathogen Fusarium graminearum Based on Genetic and Physical Mapping and Cytological Observations Genetics, November 1, 2005; 171(3): 985 - 1001. [Abstract] [Full Text] [PDF]

				J. F. Leslie, B. A. Summerell, S. Bullock, and F. J. Doe Description of Gibberella sacchari and neotypification of its anamorph Fusarium sacchari Mycologia, May 1, 2005; 97(3): 718 - 724. [Abstract] [Full Text] [PDF]

				J.-E. Kim, K.-H. Han, J. Jin, H. Kim, J.-C. Kim, S.-H. Yun, and Y.-W. Lee Putative Polyketide Synthase and Laccase Genes for Biosynthesis of Aurofusarin in Gibberella zeae Appl. Envir. Microbiol., April 1, 2005; 71(4): 1701 - 1708. [Abstract] [Full Text] [PDF]

				Z. Kerenyi, A. Moretti, C. Waalwijk, B. Olah, and L. Hornok Mating Type Sequences in Asexually Reproducing Fusarium Species Appl. Envir. Microbiol., August 1, 2004; 70(8): 4419 - 4423. [Abstract] [Full Text] [PDF]

				J. F. Leslie, K. A. Zeller, A. Logrieco, G. Mule, A. Moretti, and A. Ritieni Species Diversity of and Toxin Production by Gibberella fujikuroi Species Complex Strains Isolated from Native Prairie Grasses in Kansas Appl. Envir. Microbiol., April 1, 2004; 70(4): 2254 - 2262. [Abstract] [Full Text] [PDF]

				A. E. Desjardins, D. W. Brown, S.-H. Yun, R. H. Proctor, T. Lee, R. D. Plattner, S.-W. Lu, and B. G. Turgeon Deletion and Complementation of the Mating Type (MAT) Locus of the Wheat Head Blight Pathogen Gibberella zeae Appl. Envir. Microbiol., April 1, 2004; 70(4): 2437 - 2444. [Abstract] [Full Text] [PDF]

				K. A. Zeller, B. A. Summerell, S. Bullock, and J. F. Leslie Gibberella konza (Fusarium konzum) sp. nov. from prairie grasses, a new species in the Gibberella fujikuroi species complex Mycologia, September 1, 2003; 95(5): 943 - 954. [Abstract] [Full Text] [PDF]

				H. Britz, E. T. Steenkamp, T. A. Coutinho, B. D. Wingfield, W. F. O. Marasas, and M. J. Wingfield Two new species of Fusarium section Liseola associated with mango malformation Mycologia, July 1, 2002; 94(4): 722 - 730. [Abstract] [Full Text] [PDF]

				J. E. Jurgenson, K. A. Zeller, and J. F. Leslie Expanded Genetic Map of Gibberella moniliformis (Fusarium verticillioides) Appl. Envir. Microbiol., April 1, 2002; 68(4): 1972 - 1979. [Abstract] [Full Text] [PDF]

				J. E. Jurgenson, R. L. Bowden, K. A. Zeller, J. F. Leslie, N. J. Alexander, and R. D. Plattner A Genetic Map of Gibberella zeae (Fusarium graminearum) Genetics, April 1, 2002; 160(4): 1451 - 1460. [Abstract] [Full Text] [PDF]

				F. Trail, H. Xu, R. Loranger, and D. Gadoury Physiological and environmental aspects of ascospore discharge in Gibberella zeae (anamorph Fusarium graminearum) Mycologia, March 1, 2002; 94(2): 181 - 189. [Abstract] [Full Text] [PDF]

				S. N. Chulze, M. L. Ramirez, A. Torres, and J. F. Leslie Genetic Variation in Fusarium Section Liseola from No-Till Maize in Argentina Appl. Envir. Microbiol., December 1, 2000; 66(12): 5312 - 5315. [Abstract] [Full Text]

				A. E. Desjardins, H. K. Manandhar, R. D. Plattner, G. G. Manandhar, S. M. Poling, and C. M. Maragos Fusarium Species from Nepalese Rice and Production of Mycotoxins and Gibberellic Acid by Selected Species Appl. Envir. Microbiol., March 1, 2000; 66(3): 1020 - 1025. [Abstract] [Full Text]

				Z. Kerényi, K. Zeller, L. Hornok, and J. F. Leslie Molecular Standardization of Mating Type Terminology in the Gibberella fujikuroi Species Complex Appl. Envir. Microbiol., September 1, 1999; 65(9): 4071 - 4076. [Abstract] [Full Text]

				E. T. Steenkamp, B. D. Wingfield, T. A. Coutinho, M. J. Wingfield, and W. F. O. Marasas Differentiation of Fusarium subglutinans f. sp. pini by Histone Gene Sequence Data Appl. Envir. Microbiol., August 1, 1999; 65(8): 3401 - 3406. [Abstract] [Full Text]

				H. Britz, T. A. Coutinho, M. J. Wingfield, W. F. O. Marasas, T. R. Gordon, and J. F. Leslie Fusarium subglutinans f. sp. pini Represents a Distinct Mating Population in the Gibberella fujikuroi Species Complex Appl. Envir. Microbiol., March 1, 1999; 65(3): 1198 - 1201. [Abstract] [Full Text]

				C. J. Kedera, R. D. Plattner, and A. E. Desjardins Incidence of Fusarium spp. and Levels of Fumonisin B1 in Maize in Western Kenya Appl. Envir. Microbiol., January 1, 1999; 65(1): 41 - 44. [Abstract] [Full Text]

				G. Munkvold, H. M. Stahr, A. Logrieco, A. Moretti, and A. Ritieni Occurrence of Fusaproliferin and Beauvericin in Fusarium-Contaminated Livestock Feed in Iowa Appl. Envir. Microbiol., October 1, 1998; 64(10): 3923 - 3926. [Abstract] [Full Text]

				H. Britz, M. J. Wingfield, T. A. Coutinho, W. F. O. Marasas, and J. F. Leslie Female Fertility and Mating Type Distribution in a South African Population of Fusarium subglutinans f. sp. pini Appl. Envir. Microbiol., June 1, 1998; 64(6): 2094 - 2095. [Abstract] [Full Text]