Genetics, Vol 118, 401-410, Copyright © 1988

INVESTIGATIONS

Physical Lengths of Meiotic and Mitotic Gene Conversion Tracts in Saccharomyces cerevisiae

S. R. Judd and T. D. Petes
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637

Physical lengths of gene conversion tracts for meiotic and mitotic conversions were examined, using the same diploid yeast strain in all experiments. This strain is heterozygous for a mutation in the URA3 gene as well as closely linked restriction site markers. In cells that had a gene conversion event at the URA3 locus, it was determined by Southern analysis which of the flanking heterozygous restriction sites had co-converted. It was found that mitotic conversion tracts were longer on the average than meiotic tracts. About half of the tracts generated by spontaneous mitotic gene conversion included heterozygous markers 4.2 kb apart; none of the meiotic conversions included these markers. Stimulation of mitotic gene conversion by ultraviolet light or methylmethanesulfonate had no obvious effect on the size or distribution of the tracts. Almost all conversion tracts were continuous.

This article has been cited by other articles:

				S. J. Radford, M. M. Sabourin, S. McMahan, and J. Sekelsky Meiotic Recombination in Drosophila Msh6 Mutants Yields Discontinuous Gene Conversion Tracts Genetics, May 1, 2007; 176(1): 53 - 62. [Abstract] [Full Text] [PDF]

				M. A. McMurray and D. E. Gottschling An Age-Induced Switch to a Hyper-Recombinational State Science, September 26, 2003; 301(5641): 1908 - 1911. [Abstract] [Full Text] [PDF]

				P. J. Yeadon, L. Y. Koh, F. J. Bowring, J. P. Rasmussen, and D. E. A. Catcheside Recombination at his-3 in Neurospora Declines Exponentially With Distance from the Initiator, cog Genetics, October 1, 2002; 162(2): 747 - 753. [Abstract] [Full Text] [PDF]

				F. Malagon and A. Aguilera Yeast spt6-140 Mutation, Affecting Chromatin and Transcription, Preferentially Increases Recombination in Which Rad51p-Mediated Strand Exchange Is Dispensable Genetics, June 1, 2001; 158(2): 597 - 611. [Abstract] [Full Text] [PDF]

				L. Signon, A. Malkova, M. L. Naylor, H. Klein, and J. E. Haber Genetic Requirements for RAD51- and RAD54-Independent Break-Induced Replication Repair of a Chromosomal Double-Strand Break Mol. Cell. Biol., March 15, 2001; 21(6): 2048 - 2056. [Abstract] [Full Text]

				J. A. Nickoloff, D. B. Sweetser, J. A. Clikeman, G. J. Khalsa, and S. L. Wheeler Multiple Heterologies Increase Mitotic Double-Strand Break-Induced Allelic Gene Conversion Tract Lengths in Yeast Genetics, October 1, 1999; 153(2): 665 - 679. [Abstract] [Full Text] [PDF]

				F. Paques and J. E. Haber Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 349 - 404. [Abstract] [Full Text] [PDF]

				A. Nunes, V. Thathy, T. Bruderer, A. A. Sultan, R. S. Nussenzweig, and R. Menard Subtle Mutagenesis by Ends-in Recombination in Malaria Parasites Mol. Cell. Biol., April 1, 1999; 19(4): 2895 - 2902. [Abstract] [Full Text] [PDF]

				W. Chen and S. Jinks-Robertson The Role of the Mismatch Repair Machinery in Regulating Mitotic and Meiotic Recombination Between Diverged Sequences in Yeast Genetics, April 1, 1999; 151(4): 1299 - 1313. [Abstract] [Full Text]

				W. Chen and S. Jinks-Robertson Mismatch Repair Proteins Regulate Heteroduplex Formation during Mitotic Recombination in Yeast Mol. Cell. Biol., November 1, 1998; 18(11): 6525 - 6537. [Abstract] [Full Text]

				W. Hu, M. C. P. Timmermans, and J. Messing Interchromosomal Recombination in Zea mays Genetics, November 1, 1998; 150(3): 1229 - 1237. [Abstract] [Full Text]

				Evidence for Independent Mismatch Repair Processing on Opposite Sides of a Double-Strand Break in Saccharomyces cerevisiae Genetics, January 1, 1998; 148(1): 59 - 70.

				Long, Interrupted Conversion Tracts Initiated by cog in Neurospora crassa Genetics, January 1, 1998; 148(1): 113 - 122.