Interactions and Developmental Effects of Mutations in the Broad-Complex of Drosophila melanogaster

INVESTIGATIONS

Interactions and Developmental Effects of Mutations in the Broad-Complex of Drosophila melanogaster

I. Kiss, A. H. Beaton, J. Tardiff, D. Fristrom and J. W. Fristrom
Current address: Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, pf521 Hungary.

The 2B5 region on the X chromosome of Drosophila melanogaster forms an early ecdysone puff at the end of the third larval instar. The region contains a complex genetic locus, the Broad-Complex (BR-C) composed of four groups of fully complementing (br, rbp, l(1)2Bc, and l(1)2Bd) alleles, and classes of noncomplementing (npr1) and partially noncomplementing l(1)2Bab alleles. BR-C mutants prevent metamorphosis, including the morphogenesis of imaginal discs. Results are presented that indicate that the BR-C contains two major functional domains. One, the br domain is primarily, if not exclusively, involved in the elongation and eversion of appendages by imaginal discs. The second, the l(1)2Bc domain, is primarily involved in the fusion of discs to form a continuous adult epidermis. Nonetheless, the two domains may encode products with related functions because in some situations mutants in both domains appear to affect similar developmental processes.

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				X. Zhou, B. Zhou, J. W. Truman, and L. M. Riddiford Overexpression of broad: a new insight into its role in the Drosophila prothoracic gland cells J. Exp. Biol., March 1, 2004; 207(7): 1151 - 1161. [Abstract] [Full Text] [PDF]

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				C. A. Bayer, S. R. Halsell, J. W. Fristrom, D. P. Kiehart, and L. von Kalm Genetic Interactions Between the RhoA and Stubble-stubbloid Loci Suggest a Role for a Type II Transmembrane Serine Protease in Intracellular Signaling During Drosophila Imaginal Disc Morphogenesis Genetics, November 1, 2003; 165(3): 1417 - 1432. [Abstract] [Full Text] [PDF]

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				S. R. Halsell and D. P. Kiehart Second-Site Noncomplementation Identifies Genomic Regions Required for Drosophila Nonmuscle Myosin Function During Morphogenesis Genetics, April 1, 1998; 148(4): 1845 - 1863. [Abstract] [Full Text] [PDF]

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				C. Brennan, M Ashburner, and K Moses Ecdysone pathway is required for furrow progression in the developing Drosophila eye Development, January 7, 1998; 125(14): 2653 - 2664. [Abstract] [PDF]

				P. D'Avino and C. Thummel crooked legs encodes a family of zinc finger proteins required for leg morphogenesis and ecdysone-regulated gene expression during Drosophila metamorphosis Development, January 5, 1998; 125(9): 1733 - 1745. [Abstract] [PDF]

				J. C. Fletcher, P. P. D'Avino, and C. S. Thummel A steroid-triggered switch in E74 transcription factor isoforms regulates the timing of secondary-response gene expression PNAS, April 29, 1997; 94(9): 4582 - 4586. [Abstract] [Full Text] [PDF]

				W. Deng and M Bownes Two signalling pathways specify localised expression of the Broad-Complex in Drosophila eggshell patterning and morphogenesis Development, January 11, 1997; 124(22): 4639 - 4647. [Abstract] [PDF]

				G. Lam, C Jiang, and C. Thummel Coordination of larval and prepupal gene expression by the DHR3 orphan receptor during Drosophila metamorphosis Development, January 5, 1997; 124(9): 1757 - 1769. [Abstract] [PDF]

				G Gonzy-Treboul, J A Lepesant, and J Deutsch Enhancer-trap targeting at the Broad-Complex locus of Drosophila melanogaster. Genes & Dev., May 1, 1995; 9(9): 1137 - 1148. [Abstract] [PDF]

				R. Hodgetts, W. Clark, S. O'Keefe, M Schouls, K Crossgrove, G. Guild, and L von Kalm Hormonal induction of Dopa decarboxylase in the epidermis of Drosophila is mediated by the Broad-Complex Development, January 11, 1995; 121(11): 3913 - 3922. [Abstract] [PDF]

				A. Andres and C. Thummel The Drosophila 63F early puff contains E63-1, an ecdysone-inducible gene that encodes a novel Ca(2+)-binding protein Development, January 8, 1995; 121(8): 2667 - 2679. [Abstract] [PDF]

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				J. Fletcher, K. Burtis, D. Hogness, and C. Thummel The Drosophila E74 gene is required for metamorphosis and plays a role in the polytene chromosome puffing response to ecdysone Development, January 5, 1995; 121(5): 1455 - 1465. [Abstract] [PDF]