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Genetics, Vol 118, 21-29, Copyright © 1988
INVESTIGATIONS |
DNA Sequence Determinants of {lambda} Repressor Binding in Vivo
N. Benson, P. Sugiono and P. Youderian
Department of Biological Sciences, University of Southern California, Los Angeles, California 90089-1481
The critical operator determinants for {lambda} repressor recognition have been defined by analyzing the binding of wild-type repressor to a set of mutant operators in vivo. Base pair substitutions at six positions within the {lambda} operator half-site impair binding severely, and define these base pairs as critical for operator function. One mutant operator binds repressor better than the consensus operator, and is a superoperator. The model proposed by M. Lewis in 1983 for the binding of {lambda} repressor to its operator accurately predicts the observed operator requirements for binding in vivo, with several minor exceptions. The order of affinities of the six natural {lambda} operators has also been determined.
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