- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Raposa, S.
- Articles by Fox, M. S.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Raposa, S.
- Articles by Fox, M. S.
Some Features of Base Pair Mismatch and Heterology Repair in Escherichia coli
Susan Raposa 1 and Maurice S. Fox 1
1 Department of Biology, Massachusetts Institute of Technology,
Cambridge, Massachusetts 02139
We have used artificially constructed heteroallelic heteroduplex molecules of bacteriophage lambda DNA to transfect Escherichia coli, and E. coli mutants deficient in various functions involved in the adenine methylation-directed mismatch repair system, MutL, MutS, MutH, and UvrD (MutU). Analysis of the allele content of single infective centers shows that this repair system often acts on several mismatches, separated by as many as 2000 bp, on one of the strands of a heteroduplex molecule. When the methyl-directed mismatch repair system is disabled by mutH or uvrD mutations, localized mismatch repair becomes prominent. This prominent localized repair that can result in separation of very closely linked markers requires the functions MutL and MutS, is independent of adenine methylation, and appears to reflect another mechanism of mismatch repair. Heterology-containing heteroduplex molecules with a deletion in one strand often escape processing. However, when the heterology includes the stem and loop structure of a transposon, Tn10, the transposon is lost.
Submitted on April 19, 1987Accepted on July 18, 1987
This article has been cited by other articles:
 |
|
 |
|
  V. G. Godoy and M. S. Fox Transposon stability and a role for conjugational transfer in adaptive mutability PNAS, June 6, 2000; (2000) 130186597. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. S. Harris, G. Feng, K. J. Ross, R. Sidhu, C. Thulin, S. Longerich, S. K. Szigety, M. E. Winkler, and S. M. Rosenberg Mismatch repair protein MutL becomes limiting during stationary-phase mutation Genes & Dev., September 15, 1997; 11(18): 2426 - 2437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. G. Godoy and M. S. Fox Transposon stability and a role for conjugational transfer in adaptive mutability PNAS, June 20, 2000; 97(13): 7393 - 7398. [Abstract] [Full Text] [PDF]
|
|