- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Mackay, W. J.
- Articles by O'Donnell, J. M.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Mackay, W. J.
- Articles by O'Donnell, J. M.
A GENETIC ANALYSIS OF THE PTERIDINE BIOSYNTHETIC ENZYME, GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE, IN DROSOPHILA MELANOGASTER
William J. Mackay 1 and Janis M. O'Donnell 1
1 Department of Biological Sciences, Carnegie-Mellon University,
Pittsburgh, Pennsylvania 15213
Strains with mutant eye color were surveyed for levels of GTP cyclohydrolase (GTP CH), the first enzyme acting in the biosynthesis of pteridines, the pigments causing red eye color in Drosophila. Six strains were found to have reduced GTP CH activity. In five of the six strains, the reduction of activity is apparent only in the adult head of homozygous mutants. We show that mutations in Punch (2-97, Pu) have severe effects on GTP CH activity. In most cases, the reduction of activity is apparent in all tissues and stages that express the enzyme. The activity of GTP CH is shown to be closely correlated with the number of Pu+ genes in the genome. One ethyl methanesulfonate (EMS)-induced Pu mutant has a GTP CH enzyme that is unstable when compared with the wild-type enzyme. Mutations in Pu fall into three general classes. The largest class has a recessive lethal and eye color phenotype, 50% or higher GTP CH activity in heterozygotes, and equivalent defects in all tissues. A second class is dominant in eye color phenotype and recessive lethal, with less than 50% GTP CH activity in heterozygotes. The third class is homozygous viable and has severe reduction of activity in the adult head, but no or less severe loss in other tissues.
Submitted on December 24, 1982Accepted on May 5, 1983
This article has been cited by other articles:
 |
|
 |
|
  K. M. Bowling, Z. Huang, D. Xu, F. Ferdousy, C. D. Funderburk, N. Karnik, W. Neckameyer, and J. M. O'Donnell Direct Binding of GTP Cyclohydrolase and Tyrosine Hydroxylase: REGULATORY INTERACTIONS BETWEEN KEY ENZYMES IN DOPAMINE BIOSYNTHESIS J. Biol. Chem., November 14, 2008; 283(46): 31449 - 31459. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Chaudhuri, K. Bowling, C. Funderburk, H. Lawal, A. Inamdar, Z. Wang, and J. M. O'Donnell Interaction of Genetic and Environmental Factors in a Drosophila Parkinsonism Model J. Neurosci., March 7, 2007; 27(10): 2457 - 2467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Funderburk, K. M. Bowling, D. Xu, Z. Huang, and J. M. O'Donnell A typical N-terminal Extensions Confer Novel Regulatory Properties on GTP Cyclohydrolase Isoforms in Drosophila melanogaster J. Biol. Chem., November 3, 2006; 281(44): 33302 - 33312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Biggs, N Tripoulas, E Hersperger, C Dearolf, and A Shearn Analysis of the lethal interaction between the prune and Killer of prune mutations of Drosophila. Genes & Dev., October 1, 1988; 2(10): 1333 - 1343. [Abstract] [PDF]
|
|